Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kashiwagi, K.; Innami, A.; Zenda, R.; Tomitori, H.; Igarashi, K.
    The ATPase activity and the functional domain of PotA, a component of the sermidine-preferential uptake system in Escherichia coli (2002), J. Biol. Chem., 277, 24212-24219.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.11 D172N potA subunit, inactive mutant Escherichia coli
7.6.2.11 E297D potA subunit, spermidine uptake activity is greately reduced Escherichia coli
7.6.2.11 E297K potA subunit, spermidine uptake activity is reduced Escherichia coli
7.6.2.11 E297Q potA subunit, spermidine uptake activity is greately reduced Escherichia coli
7.6.2.11 F27L potA subunit, spermidine uptake activity is reduced Escherichia coli
7.6.2.11 F47L potA subunit, spermidine uptake activity is reduced Escherichia coli
7.6.2.11 L60F potA subunit, spermidine uptake activity is reduced Escherichia coli
7.6.2.11 L76P spermidine uptake activity is reduced Escherichia coli
7.6.2.11 additional information construction of mutants truncated at the carboxylic terminal domain of the potA subunit, these mutants have reduced activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.11 spermidine non competitive to ATP, acting as a feedback inhibitor of the spermidine transport Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.11 0.318
-
 ATP ATPase activity of the mutant L76P potA subunit Escherichia coli
7.6.2.11 0.39
-
 ATP ATPase activity of the potA subunit Escherichia coli
7.6.2.11 0.481
-
 ATP ATPase activity of the mutant E297Q potA subunit Escherichia coli
7.6.2.11 0.633
-
 ATP ATPase activity of the mutant E297K potA subunit Escherichia coli
7.6.2.11 0.855
-
 ATP ATPase activity of the mutant E297D potA subunit Escherichia coli
7.6.2.11 1.49
-
 ATP ATPase activity of the potABC complex Escherichia coli
7.6.2.11 2
-
 ATP ATPase activity of the mutant F47L potA subunit Escherichia coli
7.6.2.11 2.6
-
 ATP ATPase activity of the mutant L60F potA subunit Escherichia coli
7.6.2.11 7
-
 ATP ATPase activity of the mutant F27L potA subunit Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.11 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.11 ATP + H2O + spermidine/out the spermidine-preferential system, encoded by the genes potFGHI, can trasport both spermidine and putrescine Escherichia coli ADP + phosphate + spermidine/in
-
 ?