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Literature summary extracted from

  • Yang, H.; Slupska, M.M.; Wei, Y.F.; Tai, J.H.; Luther, W.M.; Xia, Y.R.; Shih, D.M.; Chiang, J.H.; Baikkalov, C.; Fitz-Gibbon, S.; Phan, I.T.; Conrad, A.; Miller, J.H.
    Cloning and characterization of a new member of nudix hydrolases from human and mouse (2000), J. Biol. Chem., 275, 8844-8853.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 hexahistidine-tagged recombinant enzyme expressed in Escherichia coli Mus musculus
3.6.1.13 hexahistidine-tagged recombinant enzyme expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.032
-
 ADP-ribose pH 7.0, 37°C Homo sapiens
3.6.1.13 0.038
-
 ADP-ribose pH 9.0, 37°C Mus musculus
3.6.1.13 0.083
-
 ADP-mannose pH 7.0, 37°C Homo sapiens
3.6.1.13 0.154
-
 ADP-mannose pH 9.0, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ 1 mM required for optimal activity Homo sapiens
3.6.1.13 Mg2+ 5 mM required for optimal activity Mus musculus
3.6.1.13 Mn2+ can partially substitute for Mg2+ Mus musculus
3.6.1.13 Mn2+ can partially substitute for Mg2+ Homo sapiens
3.6.1.13 Zn2+ can partially substitute for Mg2+ Mus musculus
3.6.1.13 Zn2+ can partially substitute for Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.13 40000
-
 x * 40000, SDS-PAGE Mus musculus
3.6.1.13 40000
-
 x * 40000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Homo sapiens Q9UKK9
-
-
3.6.1.13 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 recombinant enzyme Mus musculus
3.6.1.13 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.13 A-549 cell
-
 Homo sapiens
-
3.6.1.13 adrenal gland
-
 Homo sapiens
-
3.6.1.13 amygdala
-
 Homo sapiens
-
3.6.1.13 bone marrow
-
 Homo sapiens
-
3.6.1.13 brain
-
 Mus musculus
-
3.6.1.13 brain
-
 Homo sapiens
-
3.6.1.13 Burkitt lymphoma cell Raji and Daudi Homo sapiens
-
3.6.1.13 caudate nucleus
-
 Homo sapiens
-
3.6.1.13 caudate putamen
-
 Homo sapiens
-
3.6.1.13 cerebellum
-
 Homo sapiens
-
3.6.1.13 cerebral cortex
-
 Homo sapiens
-
3.6.1.13 colon
-
 Homo sapiens
-
3.6.1.13 duodenum
-
 Homo sapiens
-
3.6.1.13 esophagus
-
 Homo sapiens
-
3.6.1.13 frontal lobe
-
 Homo sapiens
-
3.6.1.13 heart
-
 Mus musculus
-
3.6.1.13 HeLa cell S3 Homo sapiens
-
3.6.1.13 hippocampus
-
 Homo sapiens
-
3.6.1.13 HL-60 cell
-
 Homo sapiens
-
3.6.1.13 ileocecum
-
 Homo sapiens
-
3.6.1.13 ileum
-
 Homo sapiens
-
3.6.1.13 jejunum
-
 Homo sapiens
-
3.6.1.13 K-562 cell
-
 Homo sapiens
-
3.6.1.13 kidney
-
 Mus musculus
-
3.6.1.13 kidney
-
 Homo sapiens
-
3.6.1.13 leukocyte peripheral blood Homo sapiens
-
3.6.1.13 liver
-
 Mus musculus
-
3.6.1.13 liver
-
 Homo sapiens
-
3.6.1.13 lung
-
 Mus musculus
-
3.6.1.13 lung
-
 Homo sapiens
-
3.6.1.13 lymph node
-
 Homo sapiens
-
3.6.1.13 mammary gland
-
 Homo sapiens
-
3.6.1.13 medulla oblongata
-
 Homo sapiens
-
3.6.1.13 MOLT-4 cell
-
 Homo sapiens
-
3.6.1.13 occipital lobe
-
 Homo sapiens
-
3.6.1.13 pancreas
-
 Homo sapiens
-
3.6.1.13 parietal lobe
-
 Homo sapiens
-
3.6.1.13 pituitary gland
-
 Homo sapiens
-
3.6.1.13 placenta
-
 Homo sapiens
-
3.6.1.13 pons
-
 Homo sapiens
-
3.6.1.13 prostate
-
 Homo sapiens
-
3.6.1.13 rectum
-
 Homo sapiens
-
3.6.1.13 salivary gland
-
 Homo sapiens
-
3.6.1.13 skeletal muscle
-
 Mus musculus
-
3.6.1.13 spinal cord
-
 Homo sapiens
-
3.6.1.13 spleen
-
 Mus musculus
-
3.6.1.13 spleen
-
 Homo sapiens
-
3.6.1.13 stomach
-
 Homo sapiens
-
3.6.1.13 substantia nigra weak Homo sapiens
-
3.6.1.13 SW-480 cell
-
 Homo sapiens
-
3.6.1.13 testis
-
 Mus musculus
-
3.6.1.13 testis
-
 Homo sapiens
-
3.6.1.13 thalamus weak Homo sapiens
-
3.6.1.13 thymus
-
 Homo sapiens
-
3.6.1.13 thyroid gland
-
 Homo sapiens
-
3.6.1.13 trachea
-
 Homo sapiens
-
3.6.1.13 vermiform appendix
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-glucose + H2O about 70% of the activity with ADP-mannose Homo sapiens AMP + alpha-D-glucose 1-phosphate
-
 ?
3.6.1.13 ADP-glucose + H2O about 70% of the activity with ADP-ribose Mus musculus AMP + alpha-D-glucose 1-phosphate
-
 ?
3.6.1.13 ADP-mannose + H2O
-
 Homo sapiens AMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 ADP-mannose + H2O about 80% of the activity with ADP-ribose Mus musculus AMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O
-
 Mus musculus AMP + ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O about 65% of the activity with ADP-mannose Homo sapiens AMP + ribose 5-phosphate
-
 ?
3.6.1.13 GDP-glucose + H2O about 15% of the activity with ADP-mannose Homo sapiens GMP + glucose 5-phosphate
-
 ?
3.6.1.13 GDP-glucose + H2O about 15% of the activity with ADP-ribose Mus musculus GMP + glucose 5-phosphate
-
 ?
3.6.1.13 GDP-mannose + H2O about 15% of the activity with ADP-mannose Homo sapiens GMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 GDP-mannose + H2O about 20% of the activity with ADP-ribose Mus musculus GMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 NADH + H2O about 12% of the activity with ADP-mannose Homo sapiens AMP + NMNH
-
 ?
3.6.1.13 NADH + H2O about 15% of the activity with ADP-ribose Mus musculus AMP + NMNH
-
 ?
3.6.1.13 UDP-glucose + H2O about 12% of the activity with ADP-mannose Homo sapiens UMP + glucose 5-phosphate
-
 ?
3.6.1.13 UDP-glucose + H2O about 5% of the activity with ADP-ribose Mus musculus UMP + glucose 5-phosphate
-
 ?
3.6.1.13 UDP-mannose + H2O about 15% of the activity with ADP-mannose Homo sapiens UMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 UDP-mannose + H2O about 5% of the activity with ADP-ribose Mus musculus UMP + D-mannose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.13 ? x * 40000, SDS-PAGE Mus musculus
3.6.1.13 ? x * 40000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 ADP-ribose pyrophosphatase
-
 Mus musculus
3.6.1.13 ADP-ribose pyrophosphatase
-
 Homo sapiens
3.6.1.13 mNUDT5 protein
-
 Mus musculus
3.6.1.13 NUDT5 protein
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 6.5 9 activity at pH 8.0 is about 90% of the activity at pH 6.5 and at pH 9.0, activity with ADP-ribose Homo sapiens
3.6.1.13 7
-
 activity with ADP-mannose Homo sapiens
3.6.1.13 9
-
 activity with ADP-mannose or ADP-ribose Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.13 4.5 10 pH 4.5: about 55% of maximal activity, pH 10.0: about 50% of maximal activity, activity with ADP-mannose Homo sapiens
3.6.1.13 6 10 pH 6.0: about 25% of maximal activity, pH 10.0: about 60% of maximal activity, activity with ADP-ribose Homo sapiens
3.6.1.13 7.5 10 pH 7.5: about 60% of maximal activity with ADP-ribose, about 50% of maximal activity with ADP-mannose, pH 10.0: about 70% of maximal activity with activity with ADP-ribose or ADP-mannose Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.13 Mus musculus
-
-
 4.9
3.6.1.13 Homo sapiens
-
-
 4.9