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Literature summary extracted from

  • Gao, G.J.; Fonda, M.L.
    Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes (1994), J. Biol. Chem., 269, 8234-8239.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.74 2,2'-dithiodipyridine 0.005 mM, 50% inhibition Homo sapiens
3.1.3.74 4,4'-dithiodipyridine 0.005 mM, 50% inhibition Homo sapiens
3.1.3.74 5,5'-dithiobis(2-nitrobenzoic acid) 50 nM, 50% inhibition, incorporation of 1 mol per mol of subunit leads to complete inactivation, phosphate or dithiothreitol protects Homo sapiens
3.1.3.74 disulfide reagent reactivation by excess dithiothreitol, inactivation is due to formation of a mixed disulfide between the reagent and a free cysteinyl residue at or near the active site of enzyme Homo sapiens
3.1.3.74 iodoacetate 0.075 mM, 50% inhibition, incorporation of 0.6 mol per mol of subunit leads to complete inactivation, phosphate protects, inhibition kinetics Homo sapiens
3.1.3.74 additional information not inhibited by iodoacetamide or cystamine Homo sapiens
3.1.3.74 N-ethylmaleimide 0.1 mM, 50% inhibition, incorporation of 0.6 mol per mol of subunit leads to complete inactivation, inhibition kinetics, phosphate protects Homo sapiens
3.1.3.74 p-chloromercuribenzoate 250 nM, 50% inhibition Homo sapiens
3.1.3.74 phosphate competitive inhibitor Homo sapiens
3.1.3.74 thiol-specific reagent a variety of thiol-specific reagents inactivate in a time- and concentration-dependent manner, pyridoxal phosphate or phosphate protects Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.74 Mg2+ requirement Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.74 32000
-
 2 * 32000 Homo sapiens
3.1.3.74 64000
-
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.74
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.74 erythrocyte outdated packed erythrocytes Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.74 pyridoxal 5'-phosphate + H2O best substrate, a cysteinyl residue at or near the active site is essential for activity, there may be only one free Cys per subunit Homo sapiens pyridoxal + phosphate
-
 ?
3.1.3.74 pyridoxine 5'-phosphate + H2O
-
 Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.74 dimer 2 * 32000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.74 pyridoxal-specific phosphatase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.74 6 8
-
 Homo sapiens