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Literature summary extracted from

  • Schröder, I.; Vadas, A.; Johnson, E.; Lim, S.; Monbouquette, H.G.
    A novel archaeal alanine dehydrogenase homologous to ornithine cyclodeaminase and mu-crystallin (2004), J. Bacteriol., 186, 7680-7689.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 expression in Escherichia coli Archaeoglobus fulgidus

General Stability

EC Number General Stability Organism
1.4.1.1 optimal KCl concentration for stability is 1.5 M Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Ketobutyrate
-
 Archaeoglobus fulgidus
1.4.1.1 L-2-aminobutyrate
-
 Archaeoglobus fulgidus
1.4.1.1 L-Ala
-
 Archaeoglobus fulgidus
1.4.1.1 NADH
-
 Archaeoglobus fulgidus
1.4.1.1 pyruvate
-
 Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.02
-
 NADH pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 0.085
-
 L-2-aminobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 0.16
-
 pyruvate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 0.48
-
 2-oxobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 0.6
-
 NAD+ pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 0.71
-
 L-Ala pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 0.97
-
 oxaloacetate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 17.3
-
 NH4+ pH 8.5, 82°C Archaeoglobus fulgidus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.1 soluble
-
 Archaeoglobus fulgidus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 35000
-
 2 * 35000, SDS-PAGE Archaeoglobus fulgidus
1.4.1.1 76000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 203
-
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 2-ketocaproate + NH3 + NADH
-
 Archaeoglobus fulgidus 2-aminocaproate + H2O + NAD+
-
 ?
1.4.1.1 3-fluoropyruvate + NH3 + NADH
-
 Archaeoglobus fulgidus ? + H2O + NAD+
-
 ?
1.4.1.1 ketovalerate + NH3 + NADH
-
 Archaeoglobus fulgidus 2-aminovalerate + H2O + NAD+
-
 ?
1.4.1.1 L-2-aminobutyrate + H2O + NAD+
-
 Archaeoglobus fulgidus 2-oxobutyrate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Archaeoglobus fulgidus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Asp + H2O + NAD+
-
 Archaeoglobus fulgidus oxaloacetate + NH3 + NADH
-
 r
1.4.1.1 L-Ile + H2O + NAD+
-
 Archaeoglobus fulgidus 2-oxo-3-methylpentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-Ser + H2O + NAD+
-
 Archaeoglobus fulgidus 3-hydroxypyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Thr + H2O + NAD+
-
 Archaeoglobus fulgidus 3-hydroxy-2-oxobutyrate + NH3 + NADH
-
 ?
1.4.1.1 L-Val + H2O + NAD+
-
 Archaeoglobus fulgidus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.1 phenylpyruvate + NH3 + NADH
-
 Archaeoglobus fulgidus L-phenylalanine + H2O + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 dimer 2 * 35000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 ALADH
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 82
-
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 25
-
 in presence of 1.5 M KCl stable for 3 months Archaeoglobus fulgidus
1.4.1.1 60
-
 less than 5% loss of activity after 65 h Archaeoglobus fulgidus
1.4.1.1 90
-
 half-life: 55 h in presence of 1.5 M Kcl Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.1 6.1
-
 NAD+ pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 6.1
-
 L-Ala pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 9.6
-
 L-2-aminobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 113
-
 oxaloacetate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 118
-
 NADH pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 118
-
 pyruvate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 118
-
 NH4+ pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 143
-
 2-oxobutyrate pH 8.5, 82°C Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 6.4 7.4 oxidative deamination Archaeoglobus fulgidus
1.4.1.1 7.5
-
 reductive amination Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.1 6.1 8.3 pH 6.1: about 60% of maximal activity, pH 8.3: about 60% of maximal activity, reductive amination Archaeoglobus fulgidus
1.4.1.1 6.2 8 pH 6.2: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, oxidative deamination Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+ NADP+ does not serve as coenzyme Archaeoglobus fulgidus
1.4.1.1 NADH NADPH does not serve as coenzyme Archaeoglobus fulgidus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.1 0.12
-
 NADH pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 4.7
-
 L-2-aminobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 4.8
-
 2-Ketobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 16.8
-
 L-Ala pH 8.5, 82°C Archaeoglobus fulgidus
1.4.1.1 27.8
-
 pyruvate pH 8.5, 82°C Archaeoglobus fulgidus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.1 Archaeoglobus fulgidus calculation from nucleotide sequence
-
 4.9