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Literature summary extracted from

  • Kazuoka, T.; Takigawa, S.; Arakawa, N.; Hizukuri, Y.; Muraoka, I.; Oikawa, T.; Soda, K.
    Novel psychrophilic and thermolabile L-threonine dehydrogenase from psychrophilic Cytophaga sp. strain KUC-1 (2003), J. Bacteriol., 185, 4483-4489.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103
-
Cytophaga sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 adenosine-5'-diphosphoribose
-
Cytophaga sp.
1.1.1.103 CuCl2 1 mM, 100% inhibition Cytophaga sp.
1.1.1.103 HgCl2 10 mM, 100% inhibition Cytophaga sp.
1.1.1.103 monoiodoacetate 10 mM, 100% inhibition Cytophaga sp.
1.1.1.103 N-ethylmaleimide 10 mM, 48% inhibition Cytophaga sp.
1.1.1.103 NADH competitive to NAD+, noncompetitive to L-threonine Cytophaga sp.
1.1.1.103 p-chloromercuribenzoic acid 10 mM, 44% inhibition Cytophaga sp.
1.1.1.103 pyruvate competitive to L-threonine Cytophaga sp.
1.1.1.103 ZnCl2 1 mM, 72% inhibition Cytophaga sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 3.56
-
L-threonine pH 9.0, 20°C Cytophaga sp.
1.1.1.103 3.81
-
L-threonine pH 9.0, 10°C Cytophaga sp.
1.1.1.103 5.96
-
L-threonine pH 9.0, 30°C Cytophaga sp.
1.1.1.103 11.2
-
L-threonine pH 9.0, 40°C Cytophaga sp.
1.1.1.103 19.5
-
L-threonine pH 9.0, 50°C Cytophaga sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 35000
-
4 * 35000, SDS-PAGE Cytophaga sp.
1.1.1.103 139000
-
gel filtration Cytophaga sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Cytophaga sp. Q8KZM4
-
-
1.1.1.103 Cytophaga sp. KUC-1 Q8KZM4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103
-
Cytophaga sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.103 L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ ordered bi-bi mechanism, NAD+ binds prior to L-threonine Cytophaga sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.103 91.4
-
pH 9.0, 10°C Cytophaga sp.
1.1.1.103 156
-
pH 9.0, 20°C Cytophaga sp.
1.1.1.103 284
-
pH 9.0, 30°C Cytophaga sp.
1.1.1.103 611
-
pH 9.0, 50°C Cytophaga sp.
1.1.1.103 722
-
pH 9.0, 40°C Cytophaga sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.103 -20°C, 10 mM potassium phosphate, pH 7.0, 30% sucrose, 0.1 mM NAD+, 5 mM L-threonine, several months, no loss of activity Cytophaga sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 DL-threo-3-hydroxynorvaline + NAD+ 31% the rate of L-threonine Cytophaga sp. ? + NADH
-
?
1.1.1.103 DL-threo-3-hydroxynorvaline + NAD+ 31% the rate of L-threonine Cytophaga sp. KUC-1 ? + NADH
-
?
1.1.1.103 L-threonine + 3-acetyl-pyridine adenine dinucleotide 60.6% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
1.1.1.103 L-threonine + 3-acetyl-pyridine adenine dinucleotide 60.6% the rate of NAD+ Cytophaga sp. KUC-1 L-2-amino-3-oxobutanoate + ?
-
?
1.1.1.103 L-threonine + 3-pyridinealdehyde adenine dinucleotide 7.2% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
1.1.1.103 L-threonine + 3-pyridinealdehyde adenine dinucleotide 7.2% the rate of NAD+ Cytophaga sp. KUC-1 L-2-amino-3-oxobutanoate + ?
-
?
1.1.1.103 L-threonine + NAD+
-
Cytophaga sp. (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?
1.1.1.103 L-threonine + NAD+
-
Cytophaga sp. KUC-1 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?
1.1.1.103 L-threonine + nicotinamide guanine dinucleotide 5.1% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
1.1.1.103 L-threonine + thionicotinamide-NAD+ 5.1% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
1.1.1.103 additional information specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide Cytophaga sp. ?
-
?
1.1.1.103 additional information specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide Cytophaga sp. KUC-1 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 homotetramer 4 * 35000, SDS-PAGE Cytophaga sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 45
-
-
Cytophaga sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.103 10 30 60 min, more than 90% residual activity Cytophaga sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.103 40
-
half-life 160 min Cytophaga sp.
1.1.1.103 45
-
half-life 14 min Cytophaga sp.
1.1.1.103 50
-
rapid inactivation Cytophaga sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 9.5
-
-
Cytophaga sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.103 8.5
-
74% of maximum activity Cytophaga sp.
1.1.1.103 10.5
-
31% of maximum activity Cytophaga sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 3-acetyl-pyridine adenine dinucleotide
-
Cytophaga sp.
1.1.1.103 nicotinamide guanine dinucleotide
-
Cytophaga sp.
1.1.1.103 thionicotinamide-NAD+
-
Cytophaga sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.103 0.37
-
adenosine-5'-diphosphoribose substrate NAD+, pH 9.0, 30°C Cytophaga sp.
1.1.1.103 1.42
-
adenosine-5'-diphosphoribose substrate L-threonine, pH 9.0, 30°C Cytophaga sp.
1.1.1.103 23.2
-
pyruvate substrate L-threonine, pH 9.0, 30°C Cytophaga sp.
1.1.1.103 50.7
-
pyruvate substrate NAD+, pH 9.0, 30°C Cytophaga sp.