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Literature summary extracted from

  • Stabb, E.V.; Reich, K.A.; Ruby, E.G.
    Vibrio fischeri genes hvnA and hvnB encode secreted NAD(+)-glycohydrolases (2001), J. Bacteriol., 183, 309-317.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.5 gene hvnA, part of a four gene operon, and monocistronic gene hvnB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha and BW23474 Aliivibrio fischeri

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.5 additional information construction of inactive hvnA/hvnB null mutant Aliivibrio fischeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.5 hydroxylamine inhibits the reaction between ADP-ribose and polypeptides Aliivibrio fischeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.5 extracellular secretion Aliivibrio fischeri
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.5 32000
-
 x * 32000, recombinant enzyme HvnB, SDS-PAGE Aliivibrio fischeri

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.5 Aliivibrio fischeri Q56685 isolated from Euprymna scolopes, genes hvnA and hvnB
-
3.2.2.5 Aliivibrio fischeri Q9F0R5 isolated from Euprymna scolopes light organ, genes hvnA and hvnB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.5 native enzymes partially from culture supernatant, recombinant enzymes from Escherichia coli Aliivibrio fischeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.5 1,N6-etheno-NAD+ + H2O
-
 Aliivibrio fischeri ?
-
 ?
3.2.2.5 NAD+ + H2O
-
 Aliivibrio fischeri ADP-ribose + nicotinamide ADP-ribose reacts further with polyarginine, polyhistidine, or polylysine ?
3.2.2.5 NAD+ + H2O
-
 Aliivibrio fischeri ADP-ribose + nicotinamide ADP-ribose reacts spontaneously with polyarginine, polyhistidine, or polylysine ?

Subunits

EC Number Subunits Comment Organism
3.2.2.5 ? x * 32000, recombinant enzyme HvnB, SDS-PAGE Aliivibrio fischeri

Synonyms

EC Number Synonyms Comment Organism
3.2.2.5 HvnA
-
 Aliivibrio fischeri
3.2.2.5 HvnB
-
 Aliivibrio fischeri
3.2.2.5 NAD+-glycohydrolase
-
 Aliivibrio fischeri
3.2.2.5 NADase
-
 Aliivibrio fischeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.5 30
-
 assay at Aliivibrio fischeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.5 7
-
 assay at Aliivibrio fischeri