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Literature summary extracted from

  • Seffernick, J.L.; de Souza, M.L.; Sadowsky, M.J.; Wackett, L.P.
    Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different (2001), J. Bacteriol., 183, 2405-2410.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.45 2-chloro-4,6-diamino-s-triazine up to 50% inhibition of melamine turnover Acidovorax citrulli
3.5.4.45 aminoatrazine up to 71% inhibition of melamine turnover Acidovorax citrulli
3.5.4.45 ammeline up to 50% inhibition of melamine turnover Acidovorax citrulli
3.8.1.8 aminotriazine
-
 Pseudomonas sp.
3.8.1.8 additional information no inhibition by melamine and 2-chloro-4,6-diamino-S-triazine Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.8 atrazine + H2O Pseudomonas sp. specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ?
3.8.1.8 additional information Pseudomonas sp. no activity with melamine and ammeline ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.45 Acidovorax citrulli Q9EYU0
-
-
3.5.4.45 Acidovorax citrulli NRRL B-12227 Q9EYU0
-
-
3.8.1.8 Pseudomonas sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.8.1.8 additional information inducible by atrazine Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.45 0.017
-
 substrate 2-chloro-4,6-diamino-s-triazine, pH not specified in the publication, 22°C Acidovorax citrulli
3.5.4.45 0.017
-
 substrate aminoatrazine, pH not specified in the publication, 22°C Acidovorax citrulli
3.5.4.45 0.135
-
 substrate ammeline, pH not specified in the publication, 22°C Acidovorax citrulli
3.5.4.45 0.245
-
 substrate melamine, pH not specified in the publication, 22°C Acidovorax citrulli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.45 2-chloro-4,6-diamino-s-triazine + H2O
-
 Acidovorax citrulli 2-chloro-4-amino-6-hydroxy-s-triazine + NH3
-
 ?
3.5.4.45 2-chloro-4,6-diamino-s-triazine + H2O
-
 Acidovorax citrulli NRRL B-12227 2-chloro-4-amino-6-hydroxy-s-triazine + NH3
-
 ?
3.5.4.45 aminoatrazine + H2O
-
 Acidovorax citrulli hydroxyatrazine + NH3
-
 ?
3.5.4.45 aminoatrazine + H2O
-
 Acidovorax citrulli NRRL B-12227 hydroxyatrazine + NH3
-
 ?
3.5.4.45 ammeline + H2O
-
 Acidovorax citrulli ammelide + NH3
-
 ?
3.5.4.45 ammeline + H2O
-
 Acidovorax citrulli NRRL B-12227 ammelide + NH3
-
 ?
3.5.4.45 melamine + H2O
-
 Acidovorax citrulli ammeline + NH3
-
 ?
3.5.4.45 melamine + H2O
-
 Acidovorax citrulli NRRL B-12227 ammeline + NH3
-
 ?
3.5.4.45 additional information melamine deaminase has no detectable activity with the halo-triazine substrates. Melamine deaminase is active in deamination of a substrate that is structurally identical to atrazine, except for the substitution of an amino agroup for the chlorine atom. No substrates: ammelide, atrazine, desethylatrazine, desisopropylatrazine, cyromazine, fluoroatrazine, azidoatrazine, atratone, cyanoatrazine, 2,4,6-triaminopyrimidine, 2-amino-4,6-dichloropyrimidine, 6-chloro-2,4-diaminopyrimidine Acidovorax citrulli ?
-
 ?
3.5.4.45 additional information melamine deaminase has no detectable activity with the halo-triazine substrates. Melamine deaminase is active in deamination of a substrate that is structurally identical to atrazine, except for the substitution of an amino agroup for the chlorine atom. No substrates: ammelide, atrazine, desethylatrazine, desisopropylatrazine, cyromazine, fluoroatrazine, azidoatrazine, atratone, cyanoatrazine, 2,4,6-triaminopyrimidine, 2-amino-4,6-dichloropyrimidine, 6-chloro-2,4-diaminopyrimidine Acidovorax citrulli NRRL B-12227 ?
-
 ?
3.8.1.8 atrazine + H2O specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine Pseudomonas sp. 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ?
3.8.1.8 atrazine + H2O specific for halo-substituted triazine ring compounds Pseudomonas sp. 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ?
3.8.1.8 additional information no activity with melamine and ammeline Pseudomonas sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.45 triA
-
 Acidovorax citrulli
3.8.1.8 AtzA
-
 Pseudomonas sp.
3.8.1.8 More enzyme shows 98% amino acid sequence identitiy with melamine deaminase from Pseudomonas sp. strain NRRL B-12227, but does not catalyse the same reaction Pseudomonas sp.