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Literature summary extracted from

  • Padmajanti, A.; Tonozuka, T.; Sakano, Y.
    Deglycosylated isopullulanase retains enzymatic activity (2000), J. Appl. Glycosci., 47, 287-292.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.57 ipuA gene, expression in Aspergillus oryzae M-2-3 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.57 additional information
-
 additional information kinetic parameters for deglycosylated recombinant IPU with a sugar content of 13.8%, 6.8% and 2.1%, respectively, deglycosylation decreases the kinetic parameters, except Km for pullulan Aspergillus niger
3.2.1.57 0.88
-
 pullulan 40°C, recombinant IPU Aspergillus niger
3.2.1.57 39.2
-
 panose 40°C, recombinant IPU Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.57 59000
-
 x * 59000, deglycosylated recombinant IPU, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.57 Aspergillus niger
-
 strain ATCC 9642
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.57 glycoprotein the sugar content of native IPU is 12.4-15.3%, that of recombinant IPU is 33.8%, deglycosylated enzyme by endoglycosidase H with a sugar content of 2.1% retains 65% of its original activity, kinetics of deglycosylated IPU, hybrid- and/or high-mannose types Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.57 panose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.57 pullulan + H2O very strict substrate specificity, preferred over panose Aspergillus niger isopanose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.57 ? x * 59000, deglycosylated recombinant IPU, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.57 IPU
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.57 35 40 deglycosylated recombinant IPU, native IPU has a similar optimum temperature Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.57 40
-
 deglycosylated recombinant IPU, stable up to 40°C, native IPU has a similar thermal stability Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.57 3.5
-
 deglycosylated recombinant IPU, native IPU has a similar pH-optimum Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.57 3 7 deglycosylated recombinant IPU, stable Aspergillus niger