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Literature summary extracted from
- Conversion of extradiol aromatic ring-cleaving homoprotocatechuate 2,3-dioxygenase into an intradiol cleaving enzyme (2003), J. Am. Chem. Soc., 125, 11780-11781.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.11.15 | H200F | switch of reaction from extradiol to intradiol cleavage for substrate 2,3-dihydroxybenzoate | Brevibacterium fuscum |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.15 | Fe2+ | - |
Brevibacterium fuscum |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.15 | Brevibacterium fuscum | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.15 | 2,3-dihydroxybenzoate + O2 | - |
Brevibacterium fuscum | CO2 + alpha-hydroxymuconic semialdehyde | mutant H200F produces alpha-carboxy-cis,cis-muconic acid | ? | |
| 1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | - |
Brevibacterium fuscum | 2-hydroxy-5-carboxymethyl-cis,cis-muconate semialdehyde | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.15 | 0.01 | - |
2,3-Dihydroxybenzoate | mutant H200F | Brevibacterium fuscum | |
| 1.13.11.15 | 0.02 | - |
2,3-Dihydroxybenzoate | wild-type | Brevibacterium fuscum | |
| 1.13.11.15 | 0.12 | - |
3,4-Dihydroxyphenylacetate | mutant H200F | Brevibacterium fuscum | |
| 1.13.11.15 | 10 | - |
3,4-Dihydroxyphenylacetate | wild-type | Brevibacterium fuscum | |
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Release 2023.1 (January 2023)
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