EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | 1-deoxynojirimycine | strong inhibition of hydrolysis of kojibiose, nigerose | Entamoeba histolytica | |
3.2.1.20 | 3-deoxy-D-glucose | strong inhibition | Entamoeba histolytica | |
3.2.1.20 | 6-deoxy-D-glucose | strong inhibition | Entamoeba histolytica | |
3.2.1.20 | castanospermine | strong inhibition of hydrolysis of kojibiose, nigerose | Entamoeba histolytica | |
3.2.1.20 | D-galactose | slight inhibition | Entamoeba histolytica | |
3.2.1.20 | D-glucose | strong inhibition | Entamoeba histolytica | |
3.2.1.20 | diethyldicarbonate | strong inhibition | Entamoeba histolytica | |
3.2.1.20 | additional information | no inhibition by australine, mannose, and 2-deoxy-D-glucose, not affected by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide | Entamoeba histolytica | |
3.2.1.20 | NEM | slight inhibition | Entamoeba histolytica | |
3.2.1.20 | phosphatidylcholine | - |
Entamoeba histolytica | |
3.2.1.20 | phosphatidylglycerol | - |
Entamoeba histolytica | |
3.2.1.20 | Tris buffer | inactivation | Entamoeba histolytica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Entamoeba histolytica | - |
- |
- |
3.2.1.20 | Entamoeba histolytica HM1:IMSS | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | partial purification by solubilzation and ion exchange chromatography, 3.8fold, further purification via concanavalin A affinity | Entamoeba histolytica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 0.0027 | - |
partially purified enzyme | Entamoeba histolytica |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.20 | 4°C, partially purified enzyme, 69% remaining activity after 10 days, 30% remaining activity after 20 days | Entamoeba histolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | 4-methylumbelliferyl alpha-D-glucopyranoside + H2O | - |
Entamoeba histolytica | 4-methylumbelliferol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | isomaltose + H2O | hydrolysis of the alpha-1,6-glycosidic bond | Entamoeba histolytica | 2 alpha-D-glucose | - |
? | |
3.2.1.20 | kojibiose + H2O | hydrolysis of the alpha-1,2-glycosidic bond | Entamoeba histolytica | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | kojibiose + H2O | hydrolysis of the alpha-1,2-glycosidic bond | Entamoeba histolytica HM1:IMSS | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | hydrolysis of the alpha-1,4-glycosidic bond | Entamoeba histolytica | 2 D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | hydrolysis of the alpha-1,4-glycosidic bond | Entamoeba histolytica HM1:IMSS | 2 D-glucose | - |
? | |
3.2.1.20 | additional information | no activity with cellobiose | Entamoeba histolytica | ? | - |
? | |
3.2.1.20 | additional information | no activity with cellobiose | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
3.2.1.20 | nigerose + H2O | best substrate, hydrolysis of the alpha-1,3-glycosidic bond | Entamoeba histolytica | 2 alpha-D-glucose | - |
? | |
3.2.1.20 | nigerose + H2O | best substrate, hydrolysis of the alpha-1,3-glycosidic bond | Entamoeba histolytica HM1:IMSS | 2 alpha-D-glucose | - |
? | |
3.2.1.20 | trehalose + H2O | hydrolysis of the alpha-1,1-glycosidic bond | Entamoeba histolytica | ? | - |
? | |
3.2.1.20 | trehalose + H2O | hydrolysis of the alpha-1,1-glycosidic bond | Entamoeba histolytica HM1:IMSS | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 45 | - |
- |
Entamoeba histolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 6.5 | - |
- |
Entamoeba histolytica |