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Literature summary extracted from

  • Bravo-Torres, J.C.; Villagomez-Castro, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.
    Purification and biochemical characterisation of a membrane-bound alpha-glucosidase from the parasite Entamoeba histolytica (2004), Int. J. Parasitol., 34, 455-462.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 1-deoxynojirimycine strong inhibition of hydrolysis of kojibiose, nigerose Entamoeba histolytica
3.2.1.20 3-deoxy-D-glucose strong inhibition Entamoeba histolytica
3.2.1.20 6-deoxy-D-glucose strong inhibition Entamoeba histolytica
3.2.1.20 castanospermine strong inhibition of hydrolysis of kojibiose, nigerose Entamoeba histolytica
3.2.1.20 D-galactose slight inhibition Entamoeba histolytica
3.2.1.20 D-glucose strong inhibition Entamoeba histolytica
3.2.1.20 diethyldicarbonate strong inhibition Entamoeba histolytica
3.2.1.20 additional information no inhibition by australine, mannose, and 2-deoxy-D-glucose, not affected by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide Entamoeba histolytica
3.2.1.20 NEM slight inhibition Entamoeba histolytica
3.2.1.20 phosphatidylcholine
-
Entamoeba histolytica
3.2.1.20 phosphatidylglycerol
-
Entamoeba histolytica
3.2.1.20 Tris buffer inactivation Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Entamoeba histolytica
-
-
-
3.2.1.20 Entamoeba histolytica HM1:IMSS
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 partial purification by solubilzation and ion exchange chromatography, 3.8fold, further purification via concanavalin A affinity Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 0.0027
-
partially purified enzyme Entamoeba histolytica

Storage Stability

EC Number Storage Stability Organism
3.2.1.20 4°C, partially purified enzyme, 69% remaining activity after 10 days, 30% remaining activity after 20 days Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-methylumbelliferyl alpha-D-glucopyranoside + H2O
-
Entamoeba histolytica 4-methylumbelliferol + alpha-D-glucopyranose
-
?
3.2.1.20 isomaltose + H2O hydrolysis of the alpha-1,6-glycosidic bond Entamoeba histolytica 2 alpha-D-glucose
-
?
3.2.1.20 kojibiose + H2O hydrolysis of the alpha-1,2-glycosidic bond Entamoeba histolytica alpha-D-glucose + D-glucose
-
?
3.2.1.20 kojibiose + H2O hydrolysis of the alpha-1,2-glycosidic bond Entamoeba histolytica HM1:IMSS alpha-D-glucose + D-glucose
-
?
3.2.1.20 maltose + H2O hydrolysis of the alpha-1,4-glycosidic bond Entamoeba histolytica 2 D-glucose
-
?
3.2.1.20 maltose + H2O hydrolysis of the alpha-1,4-glycosidic bond Entamoeba histolytica HM1:IMSS 2 D-glucose
-
?
3.2.1.20 additional information no activity with cellobiose Entamoeba histolytica ?
-
?
3.2.1.20 additional information no activity with cellobiose Entamoeba histolytica HM1:IMSS ?
-
?
3.2.1.20 nigerose + H2O best substrate, hydrolysis of the alpha-1,3-glycosidic bond Entamoeba histolytica 2 alpha-D-glucose
-
?
3.2.1.20 nigerose + H2O best substrate, hydrolysis of the alpha-1,3-glycosidic bond Entamoeba histolytica HM1:IMSS 2 alpha-D-glucose
-
?
3.2.1.20 trehalose + H2O hydrolysis of the alpha-1,1-glycosidic bond Entamoeba histolytica ?
-
?
3.2.1.20 trehalose + H2O hydrolysis of the alpha-1,1-glycosidic bond Entamoeba histolytica HM1:IMSS ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45
-
-
Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6.5
-
-
Entamoeba histolytica