EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.99.30 | synthesis | enzyme can be used as a stereospecific biocatalyst for production of a wide range of compounds, e.g. 3-enoic-, 3,5-dienoic-, and 4-oxo-3R,S-hydroxyacids | Proteus vulgaris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.30 | membrane | bound | Proteus vulgaris | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.30 | Molybdenum | required | Proteus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.30 | 64000 | - |
x * 80000 + x * 64000, alpha,beta-structure | Proteus vulgaris |
1.1.99.30 | 80000 | - |
x * 80000 + x * 64000, alpha,beta-structure | Proteus vulgaris |
1.1.99.30 | 600000 | - |
above | Proteus vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.30 | Proteus vulgaris | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.99.30 | a (2R)-hydroxy-carboxylate + acceptor = a 2-oxo-carboxylate + reduced acceptor | mechanism | Proteus vulgaris |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.30 | 1000 | - |
purified enzyme | Proteus vulgaris |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.99.30 | -15°C, loss of 10% activity within 1-2 years | Proteus vulgaris |
1.1.99.30 | room temperature, freeze dried cells, loss of 10% of activity within 1-2 years | Proteus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.30 | (2S,3R,4S)-2-hydroxy-3-halogenbutyrolactones + reduced benzyl viologen | highly stereospecific reaction | Proteus vulgaris | ? | - |
r | |
1.1.99.30 | (2S,3R,4S)-2-hydroxy-3-halogenbutyrolactones + reduced methyl viologen | highly stereospecific reaction | Proteus vulgaris | ? | - |
r | |
1.1.99.30 | 2-oxo-carboxylate + reduced benzyl viologen | stereospecific, very wide substrate specificity, reduces 2-oxo-carboxylates with diverse types of residues at position 3, such as unbranched and branched alkyl, hydroxy, one or two conjugated carbon carbon double bonds, additional CO, and -OOC(CH2)n, overview | Proteus vulgaris | (2R)-hydroxy-carboxylate + oxidized benzyl viologen | - |
r | |
1.1.99.30 | 2-oxo-carboxylate + reduced methyl viologen | stereospecific, very wide substrate specificity, reduces 2-oxo-carboxylates with diverse types of residues at position 3, such as unbranched and branched alkyl, hydroxy, one or two conjugated carbon carbon double bonds, additional CO, and -OOC(CH2)n, overview | Proteus vulgaris | (2R)-hydroxy-carboxylate + oxidized methyl viologen | - |
r | |
1.1.99.30 | additional information | 2-oxo acids with diverse substituents, e.g. carboxy, methyl, ethyl, halogen, methoxy, thiomethyl, NHCHO, unbranched and branched alkyl, OH-containing residues in 3-position, double bonds containing residues, substituents with additional CO groups, -OOC(CH2)n with n being at least 3, overview | Proteus vulgaris | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.30 | oligomer | x * 80000 + x * 64000, alpha,beta-structure | Proteus vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.30 | (2R)-hydroxycarboxlate-viologen-oxidoreductase | - |
Proteus vulgaris |
1.1.99.30 | 2-oxoacid reductase | - |
Proteus vulgaris |
1.1.99.30 | D-2-hydroxy acid dehydrogenase | - |
Proteus vulgaris |
1.1.99.30 | dehydrogenase, D-2-hydroxy acid | - |
Proteus vulgaris |
1.1.99.30 | HVOR | - |
Proteus vulgaris |
1.1.99.30 | hydroxycarboxlate-viologen-oxidoreductase | - |
Proteus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.30 | 8.5 | - |
- |
Proteus vulgaris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.30 | molybdenum cofactor | i.e. MoCo | Proteus vulgaris | |
1.1.99.30 | additional information | neither NADH nor NADPH are cosubstrates for the enzyme, no flavin or heme cofactors | Proteus vulgaris |