EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | AgNO3 | 100 mM, 69% inhibition; 100 mM, 90% inhibition | Thermoascus aurantiacus | |
3.2.1.21 | CoCl2 | 100 mM, 54% inhibition | Thermoascus aurantiacus | |
3.2.1.21 | CuSO4 | 100 mM, 48% inhibition; 100 mM, 66% inhibition | Thermoascus aurantiacus | |
3.2.1.21 | FeCl3 | 100 mM, 59% inhibition | Thermoascus aurantiacus | |
3.2.1.21 | HgCl2 | 100 mM, 53% inhibition; 100 mM, 64% inhibition | Thermoascus aurantiacus | |
3.2.1.21 | KCl | 100 mM, 54% inhibition | Thermoascus aurantiacus | |
3.2.1.21 | MgSO4 | 100 mM, 44% inhibition | Thermoascus aurantiacus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 1.17 | - |
p-nitrophenyl-beta-D-glucopyranoside | 80°C, pH 4.5 | Thermoascus aurantiacus | |
3.2.1.21 | 1.38 | - |
p-nitrophenyl-beta-D-glucopyranoside | 75°C, pH 4.0 | Thermoascus aurantiacus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | Ca2+ | slight stimulation | Thermoascus aurantiacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 157000 | - |
x * 157000, SDS-PAGE | Thermoascus aurantiacus |
3.2.1.21 | 175000 | - |
x * 175000, SDS-PAGE | Thermoascus aurantiacus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Thermoascus aurantiacus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | ethanol precipitation, Sephadex G-100, Q-Sepharose | Thermoascus aurantiacus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 458.9 | - |
- |
Thermoascus aurantiacus |
3.2.1.21 | 771 | - |
- |
Thermoascus aurantiacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | p-nitrophenol + beta-D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | ? | x * 175000, SDS-PAGE | Thermoascus aurantiacus |
3.2.1.21 | ? | x * 157000, SDS-PAGE | Thermoascus aurantiacus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | Gl-2 | - |
Thermoascus aurantiacus |
3.2.1.21 | Gl-3 | - |
Thermoascus aurantiacus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 75 | - |
- |
Thermoascus aurantiacus |
3.2.1.21 | 80 | - |
- |
Thermoascus aurantiacus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 50 | 90 | approx. 25% of maximal activity at 50°C, approx. 40% of maximal activity at 90°C | Thermoascus aurantiacus |
3.2.1.21 | 50 | 90 | approx. 30% of maximal activity at 50°C, approx. 20% of maximal activity at 90°C | Thermoascus aurantiacus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 70 | - |
25% loss of activity after 1 h | Thermoascus aurantiacus |
3.2.1.21 | 70 | - |
18% loss of activity after 1 h | Thermoascus aurantiacus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 4 | - |
- |
Thermoascus aurantiacus |
3.2.1.21 | 4.5 | - |
- |
Thermoascus aurantiacus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 3 | 7 | approx. 65% of maximal activity at pH 3.0, approx. 10% of maximal activity at pH 6.0 | Thermoascus aurantiacus |
3.2.1.21 | 3 | 7 | approx. 80% of maximal activity at pH 3.0, approx. 18% of maximal activity at pH 6.0 | Thermoascus aurantiacus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 4 | 9 | no loss of activity | Thermoascus aurantiacus |
3.2.1.21 | 5.5 | 8.5 | no loss of activity | Thermoascus aurantiacus |