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Literature summary extracted from

  • Desai, N.A.; Shankar, V.
    Single-strand-specific nucleases (2003), FEMS Microbiol. Rev., 26, 457-491.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.30.1 NaCl optimal activity at 100 mM Aspergillus oryzae

General Stability

EC Number General Stability Organism
3.1.30.1 no loss of activity in the presence of 100-250 mM glyoxal Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.30.1 extracellular
-
 Aspergillus oryzae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Zn2+ zinc metalloprotein, required for activity Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 32000
-
 x * 32000 Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 RNA + H2O Aspergillus oryzae
-
 fragments of RNA
-
 ?
3.1.30.1 ssDNA + H2O Aspergillus oryzae
-
 fragments of ssDNA
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.30.1 dimethyl sulfoxide no loss of activity in 50% dimethylsulfoxide Aspergillus oryzae
3.1.30.1 dimethylformamide no loss of activity in 30% dimethylformamide Aspergillus oryzae
3.1.30.1 formaldehyde no loss of activity in 2% formaldehyde Aspergillus oryzae
3.1.30.1 formamide no loss of activity in 60% formamide Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.30.1 glycoprotein 18% carbohydrates Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 RNA + H2O
-
 Aspergillus oryzae fragments of RNA
-
 ?
3.1.30.1 ssDNA + H2O
-
 Aspergillus oryzae fragments of ssDNA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.30.1 ? x * 32000 Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 S1 nuclease
-
 Aspergillus oryzae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.30.1 Aspergillus oryzae
-
-
 4