Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mohapatra, A.; Leul, M.; Mattsson, U.; Sellstedt, A.
    A hydrogen-evolving enzyme is present in Frankia sp. R43 (2004), FEMS Microbiol. Lett., 236, 235-240.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 H2 + NAD+ Frankia sp. R43 H2 producing activity is not dependent on NAD+ reduction H+ + NADH
-
 r
1.12.1.2 H2 + oxidized methyl viologen Frankia sp. R43 the artificial electron donor is accepted by the cells for H2 production when added to the culture medium H+ + reduced methyl viologen
-
 r
1.12.1.2 additional information Frankia sp. R43 enzyme is the key enzyme in hydrogen metabolism, no activity in strain R43 under aerobic nitrogen-limited conditions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Frankia sp. R43 P22317 hoxF, alpha-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-
1.12.1.2 Frankia sp. R43 P22318 hoxU, gamma-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-
1.12.1.2 Frankia sp. R43 P22319 hoxY, delta-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-
1.12.1.2 Frankia sp. R43 P22320 hoxH, beta-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.12.1.2 hypha
-
 Frankia sp. R43
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 additional information
-
 H2-uptake activity at different conditions Frankia sp. R43
1.12.1.2 0.0006
-
 cell culture in nitrogen-enriched medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor Frankia sp. R43
1.12.1.2 0.0018
-
 cell culture in nitrogen-limited medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor Frankia sp. R43

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Frankia sp. R43 H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ H2 producing activity is not dependent on NAD+ reduction Frankia sp. R43 H+ + NADH
-
 r
1.12.1.2 H2 + oxidized methyl viologen
-
 Frankia sp. R43 H+ + reduced methyl viologen
-
 r
1.12.1.2 H2 + oxidized methyl viologen the artificial electron donor is accepted by the cells for H2 production when added to the culture medium Frankia sp. R43 H+ + reduced methyl viologen
-
 r
1.12.1.2 additional information enzyme is the key enzyme in hydrogen metabolism, no activity in strain R43 under aerobic nitrogen-limited conditions Frankia sp. R43 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.1.2 hydrogen-evolving enzyme
-
 Frankia sp. R43

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.2 27
-
 assay at, free living cell culture in vivo Frankia sp. R43

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.2 7
-
 assay at, free living cell culture in vivo Frankia sp. R43

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 additional information methyl viologen can replace NAD+/NADH Frankia sp. R43
1.12.1.2 NAD+
-
 Frankia sp. R43
1.12.1.2 NADH
-
 Frankia sp. R43