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Literature summary extracted from

  • White, E.L.; Ross, L.J.; Cunningham, A.; Escuyer, V.
    Cloning, expression, and characterization of Mycobacterium tuberculosis dihydrofolate reductase (2004), FEMS Microbiol. Lett., 232, 101-105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter Homo sapiens
1.5.1.3 expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate IC50: 1.2 nM Homo sapiens
1.5.1.3 methotrexate IC50: 8.3 nM Mycobacterium tuberculosis
1.5.1.3 trimethoprim IC50: 0.0825 mM Homo sapiens
1.5.1.3 trimethoprim IC50: 0.0165 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0028
-
 7,8-dihydrofolate pH 7.3, 37°C Homo sapiens
1.5.1.3 0.0042
-
 NADPH pH 7.3, 37°C Mycobacterium tuberculosis
1.5.1.3 0.0045
-
 7,8-dihydrofolate pH 7.3, 37°C Mycobacterium tuberculosis
1.5.1.3 0.0091
-
 NADPH pH 7.3, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 KCl maximal activity at 0.5 M Homo sapiens
1.5.1.3 KCl steady increase in activity from 0 to 1 M KCl Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 17643
-
 1 * 17643, MALDI-TOF Homo sapiens
1.5.1.3 17643
-
 1 * 17643, MALDI-TOF Mycobacterium tuberculosis
1.5.1.3 19000
-
 gel filtration Homo sapiens
1.5.1.3 19000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Homo sapiens
-
-
-
1.5.1.3 Mycobacterium tuberculosis
-
-
-
1.5.1.3 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Homo sapiens
1.5.1.3
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 15.5
-
 pH 7.0, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Mycobacterium tuberculosis 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Mycobacterium tuberculosis H37Rv 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 monomer 1 * 17643, MALDI-TOF Homo sapiens
1.5.1.3 monomer 1 * 17643, MALDI-TOF Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 5 9 activity at pH 9 is 8% of the activity at pH 5 Homo sapiens
1.5.1.3 5 9 activity at pH 9 is 8% of the activity at pH 5 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.3 0.0000012
-
 IC50: 1.2 nM Homo sapiens methotrexate
1.5.1.3 0.0000083
-
 IC50: 8.3 nM Mycobacterium tuberculosis methotrexate
1.5.1.3 0.0165
-
 IC50: 0.0165 mM Mycobacterium tuberculosis trimethoprim
1.5.1.3 0.0825
-
 IC50: 0.0825 mM Homo sapiens trimethoprim