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Literature summary extracted from

  • Iyer, G.; Chattoo, B.B.
    Purification and characterization of laccase from the rice blast fungus, Magnaporthe grisea (2003), FEMS Microbiol. Lett., 227, 121-126.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 kojic acid 3 mM, 48% inhibition Pyricularia grisea
1.10.3.2 N-hydroxylglycine 0.1 mM, 87% inhibition Pyricularia grisea
1.10.3.2 Salicylhydroxamic acid 0.08 mM, 85% inhibition Pyricularia grisea
1.10.3.2 Sodium azide 0.5 mM, 94% inhibition Pyricularia grisea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 70000
-
 x * 70000, SDS-PAGE Pyricularia grisea

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Pyricularia grisea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Pyricularia grisea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant
-
 Pyricularia grisea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 225.9
-
 30°C, pH 6.0 Pyricularia grisea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4-methylcatechol + O2 0.9% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 alpha-naphthol + O2 11.7% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 catechol + O2 2.44% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 ferulic acid + O2 13.6% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 guaiacol + O2 6.41% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 hydroquinone + O2 9.95% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 L-DOPA + O2 15.4% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 p-cresol + O2 6.35% of activity with syringaldazine Pyricularia grisea ? + H2O
-
 ?
1.10.3.2 syringaldazine + O2
-
 Pyricularia grisea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 70000, SDS-PAGE Pyricularia grisea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 1 h, 50% loss of activity Pyricularia grisea
1.10.3.2 60
-
 1 h, complete loss of activity Pyricularia grisea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6
-
 substrate syringaldazine Pyricularia grisea