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Literature summary extracted from
- Purification and characterization of a novel cellulase-free xylanase from Acrophialophora nainiana (2003), FEMS Microbiol. Lett., 223, 309-314.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.8 | 2-mercaptoethanol | - |
Acrophialophora nainiana |  |
| 3.2.1.8 | DTNB | - |
Acrophialophora nainiana | |
| 3.2.1.8 | DTT | - |
Acrophialophora nainiana | |
| 3.2.1.8 | L-cysteine | - |
Acrophialophora nainiana | |
| 3.2.1.8 | L-tryptophan | - |
Acrophialophora nainiana | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.8 | N-bromosuccimide | - |
Acrophialophora nainiana | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.8 | additional information | - |
additional information | - |
Acrophialophora nainiana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.8 | extracellular | secretion | Acrophialophora nainiana | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 27500 | - |
2 * 27500, SDS-PAGE | Acrophialophora nainiana |
| 3.2.1.8 | 54000 | - |
gel filtration | Acrophialophora nainiana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.8 | Acrophialophora nainiana | - |
XynIII | - |
| 3.2.1.8 | Acrophialophora nainiana XynIII | - |
XynIII | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | glycoprotein | - |
Acrophialophora nainiana |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.8 | from culture supernatant, 22.12fold to homogeneity by ultrafiltration and a combination of ion exchange and gel filtration chromatography | Acrophialophora nainiana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 31.25 | - |
purified enzyme | Acrophialophora nainiana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.8 | xylan + H2O | preferred substrate, soluble and insoluble substrate from oat spelt or birchwood | Acrophialophora nainiana | ? | - |
? | |
| 3.2.1.8 | xylan + H2O | preferred substrate, soluble and insoluble substrate from oat spelt or birchwood | Acrophialophora nainiana XynIII | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | dimer | 2 * 27500, SDS-PAGE | Acrophialophora nainiana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | beta-xylanase | - |
Acrophialophora nainiana |
| 3.2.1.8 | xylanase | - |
Acrophialophora nainiana |
| 3.2.1.8 | XynIII | - |
Acrophialophora nainiana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 55 | 60 | - |
Acrophialophora nainiana |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 50 | 65 | rapid decline of activity at 65°C | Acrophialophora nainiana |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 50 | - |
half-life 120 h | Acrophialophora nainiana |
| 3.2.1.8 | 60 | - |
half-life 2 h | Acrophialophora nainiana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.8 | 0.013 | - |
xylan | insoluble oat spelt xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
| 3.2.1.8 | 0.058 | - |
xylan | soluble birchwood xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
| 3.2.1.8 | 0.085 | - |
xylan | insoluble birchwood xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
| 3.2.1.8 | 0.099 | - |
xylan | soluble oat spelt xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 6.5 | - |
- |
Acrophialophora nainiana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 4 | 8.5 | 50% of maximal activity at pH 4.0 and pH 8.5 | Acrophialophora nainiana |
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