Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujishiro, K.; Uchida, H.; Shimokawa, K.; Nakano, M.; Sano, F.; Ohta, T.; Kayahara, N.; Aisaka, K.; Uwajima, T.
    Purification and properties of a new Brevibacterium sterolicum cholesterol oxidase produced by E. coli MM294/pnH10 (2002), FEMS Microbiol. Lett., 215, 243-248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.6 cholesterol oxidase II, expression in Escherichia coli MM294/pnH10 Brevibacterium sterolicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 AgNO3 cholesterol oxidase II Brevibacterium sterolicum
1.1.3.6 HgCl2 cholesterol oxidase II Brevibacterium sterolicum
1.1.3.6 PCMB cholesterol oxidase II Brevibacterium sterolicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.03
-
 cholesterol pH 7.0, 37°C, cholesterol oxidase II Brevibacterium sterolicum
1.1.3.6 1.1
-
 cholesterol pH 7.0, 37°C, cholesterol oxidase I Brevibacterium sterolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 46500
-
 cholesterol oxidase II, gel filtration Brevibacterium sterolicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Brevibacterium sterolicum
-
 nov. sp. ATCC21387
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Brevibacterium sterolicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 55.2
-
 cholesterol oxidase II Brevibacterium sterolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 beta-sitosterol + O2 about 30% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 about 55% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum ?
-
 ?
1.1.3.6 cholesterol + O2
-
 Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 dehydro-epi-androsterone + O2 about 35% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum ?
-
 ?
1.1.3.6 dehydro-epi-androsterone + O2 about 5% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum ?
-
 ?
1.1.3.6 pregnenolone + O2 about 60% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum ?
-
 ?
1.1.3.6 pregnenolone + O2 about 60% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum ?
-
 ?
1.1.3.6 stigmasterol + O2 about 10% of the activity with cholesterol, cholesterol oxidase I Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 about 10% of the activity with cholesterol, cholesterol oxidase II Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 cholesterol oxidase I
-
 Brevibacterium sterolicum
1.1.3.6 cholesterol oxidase II
-
 Brevibacterium sterolicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 55
-
 cholesterol oxidase II Brevibacterium sterolicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 50
-
 pH 7.0, 1 h, 10% loss of activity Brevibacterium sterolicum
1.1.3.6 60
-
 pH 7.0, 1 h, 82% loss of activity Brevibacterium sterolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 6.5
-
 cholesterol oxidase II Brevibacterium sterolicum
1.1.3.6 7
-
 cholesterol oxidase I Brevibacterium sterolicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 8.5 pH 5.0: about 75% of maximalm acttivity, pH 8.5: about 60% of maximal activity, cholesterol oxidase II Brevibacterium sterolicum
1.1.3.6 5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, cholesterol oxidase I Brevibacterium sterolicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 5 8 50°C, 1 h, stable Brevibacterium sterolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 flavin
-
 Brevibacterium sterolicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.6 Brevibacterium sterolicum isoelectric focusing with carrier ampholites pH 4-6, cholesterol oxidase II
-
 4.9