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Literature summary extracted from
- Purification and characterization of a new hyperthermostable, allosamidin-insensitive and denaturation-resistant chitinase from the hyperthermophilic archaeon Thermococcus chitonophagus (2003), Extremophiles, 7, 43-53.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.14 | 40% activity in presence of 1 M NaCl | Thermococcus chitonophagus |
| 3.2.1.14 | in the presence of 3 M guanidine hydrochloride no activity left | Thermococcus chitonophagus |
| 3.2.1.14 | in the presence of 3 M urea over 50% of initial activity left | Thermococcus chitonophagus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | dimethyl sulfoxide | - |
Thermococcus chitonophagus |  |
| 3.2.1.14 | guanidine hydrochloride | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | polyethyleneglycol 400 | - |
Thermococcus chitonophagus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 0.14 | - |
p-nitrophenol-beta-D-N,N'-diacetylchitobioside | 70°C, pH 7 | Thermococcus chitonophagus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.14 | membrane | associated with the outer side of cell membrane | Thermococcus chitonophagus | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 70000 | - |
1 * 70000, SDS-PAGE | Thermococcus chitonophagus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Thermococcus chitonophagus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | to homogeneity | Thermococcus chitonophagus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 0.25 | - |
p-nitrophenol-beta-D-lactopyranoside or p-nitrophenol-beta-D-galactopyranoside as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 0.35 | - |
p-nitrophenol-beta-D-cellobioside as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 0.63 | - |
p-nitrophenol-beta-D-mannopyranoside as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 0.96 | - |
p-nitrophenol-beta-D-maltoside as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 1.26 | - |
p-nitrophenol-beta-D-glucopyranoside as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 2.07 | - |
p-nitrophenol-beta-D-N,N'-diacetylchitobioside as substrate as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 230 | - |
chitosan as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 270 | - |
cellulose as substrate | Thermococcus chitonophagus |
| 3.2.1.14 | 310 | - |
chitin as substrate | Thermococcus chitonophagus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | 4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermococcus chitonophagus | 4-nitrophenol + beta-D-cellobiose | - |
? | |
| 3.2.1.14 | cellulose + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | chitin + H2O | - |
Thermococcus chitonophagus | chitobiose + ? | - |
? | |
| 3.2.1.14 | chitosan + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | N,N',N''-triacetylchitotriose + H2O | - |
Thermococcus chitonophagus | ? | - |
? | |
| 3.2.1.14 | N,N'-diacetylchitobiose + H2O | - |
Thermococcus chitonophagus | N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-cellobioside + H2O | - |
Thermococcus chitonophagus | D-cellobiose + p-nitrophenol | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-galactopyranoside + H2O | - |
Thermococcus chitonophagus | D-galactopyranose + p-nitrophenol | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermococcus chitonophagus | D-glucopyranose + p-nitrophenol | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-lactopyranoside + H2O | - |
Thermococcus chitonophagus | D-lactopyranose + p-nitrophenol | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-maltoside + H2O | - |
Thermococcus chitonophagus | D-maltose + p-nitrophenol | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-mannopyranoside + H2O | - |
Thermococcus chitonophagus | D-mannopyranose + p-nitrophenol | - |
? | |
| 3.2.1.14 | p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O | - |
Thermococcus chitonophagus | N,N'-diacetylchitobiose + p-nitrophenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | monomer | 1 * 70000, SDS-PAGE | Thermococcus chitonophagus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | Chi70 | - |
Thermococcus chitonophagus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 70 | - |
- |
Thermococcus chitonophagus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 90 | 100 | over 50% activity after 48 h | Thermococcus chitonophagus |
| 3.2.1.14 | 110 | - |
half-life of 4 h | Thermococcus chitonophagus |
| 3.2.1.14 | 120 | - |
half-life of 1 h | Thermococcus chitonophagus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 0.0025 | - |
chitosan | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.003 | - |
cellulose | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.005 | - |
chitin | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.017 | - |
p-nitrophenol-beta-D-galactopyranoside | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.025 | - |
p-nitrophenol-beta-D-lactopyranoside | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.032 | - |
p-nitrophenol-beta-D-mannopyranoside | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.04 | - |
4-nitrophenol-beta-D-cellobioside | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.07 | - |
p-nitrophenol-beta-D-maltoside | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.095 | - |
p-nitrophenol-beta-D-glucopyranoside | - |
Thermococcus chitonophagus | |
| 3.2.1.14 | 0.383 | - |
p-nitrophenol-beta-D-N,N'-diacetylchitobioside | 70°C, pH 7 | Thermococcus chitonophagus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 7 | - |
- |
Thermococcus chitonophagus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 6 | 7.5 | - |
Thermococcus chitonophagus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 3 | 9 | over 50% activity after 24 h | Thermococcus chitonophagus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.14 | Thermococcus chitonophagus | isoelectric focusing | - |
6 |
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