EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.3 | Y238F | 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 4.7fold lower than the wild-type value, Km-value for D-Pro is 1.7fold lower than the wild-type value, Km-value for D-Trp is 1.5fold lower than the wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3fold lower than the wild-type value, Km-value for D-Phe is 4.3fold lower than the wild-type value | Rhodotorula toruloides |
1.4.3.3 | Y238S | 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 8fold lower than the wild-type value, Km-value for D-Pro is 1.6fold lower than the wild-type value, Km-value for D-Trp is identical to wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3.2fold lower than the wild-type value, Km-value for D-Phe is 7.5fold lower than the wild-type value | Rhodotorula toruloides |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.3 | benzoate | competitive | Rhodotorula toruloides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 0.04 | - |
D-Phe | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 0.07 | - |
D-Phe | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 0.2 | - |
D-Trp | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 0.26 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 0.3 | - |
D-Trp | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 0.3 | - |
D-Phe | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 0.3 | - |
D-Trp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 0.96 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 1.7 | - |
D-Ser | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 1.9 | - |
cephalosporin C | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 1.9 | - |
cephalosporin C | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 2.3 | - |
O2 | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 2.6 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 2.9 | - |
D-Ser | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 5 | - |
cephalosporin C | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 6 | - |
D-Val | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 6.1 | - |
D-Val | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 7.5 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 7.8 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 12.3 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 13.5 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 13.7 | - |
D-Ser | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 18.9 | - |
D-Val | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 21.5 | - |
D-Pro | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Rhodotorula toruloides | P80324 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.3 | - |
Rhodotorula toruloides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 37 | - |
- |
Rhodotorula toruloides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Ala + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Phe + H2O + O2 | - |
Rhodotorula toruloides | phenylpyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Pro + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Ser + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Trp + H2O + O2 | - |
Rhodotorula toruloides | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Val + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoisovalerate + NH3 + H2O2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 120 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 120 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.4.3.3 | 125 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 125 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.4.3.3 | 350 | - |
O2 | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 350 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |