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Literature summary extracted from

  • Rippert, P.; Matringe, M.
    Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana (2002), Eur. J. Biochem., 269, 4753-4761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.78 TyrAAT1 overproduced in Escherichia coli Arabidopsis thaliana
1.3.1.78 TyrAAT2 overproduced in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.78 AMP
-
 Arabidopsis thaliana
1.3.1.78 cis-aconitate
-
 Arabidopsis thaliana
1.3.1.78 L-tyrosine
-
 Arabidopsis thaliana
1.3.1.78 NADPH
-
 Arabidopsis thaliana
1.3.1.78 prephenate
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.78 0.0102
-
 NADP+ pH 7.5, 25°C, TyrAAT1 Arabidopsis thaliana
1.3.1.78 0.0147
-
 NADP+ pH 7.5, 25°C, TyrAAT2 Arabidopsis thaliana
1.3.1.78 0.0526
-
 arogenate pH 7.5, 25°C, TyrAAT1 Arabidopsis thaliana
1.3.1.78 0.0842
-
 L-arogenate pH 7.5, 25°C, TyrAAT2 Arabidopsis thaliana
1.3.1.78 17
-
 prephenate
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.78 66000
-
 1 * 66000, recombinant TyrAAT1, SDS-PAGE Arabidopsis thaliana
1.3.1.78 67000
-
 recombinant TyrAAT1, gel filtration Arabidopsis thaliana
1.3.1.78 600000
-
 above, recombinant TyrAAT2 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.78 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.78 TyrAAT1 Arabidopsis thaliana
1.3.1.78 TyrAAT2 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.78 73
-
-
 Arabidopsis thaliana
1.3.1.78 142
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.78 L-arogenate + NADP+ rapid equilibrium random mechanism in which two dead-end complexes, enzyme-NADPH-arogenate and enzyme-NADP+-tyrosine are formed Arabidopsis thaliana L-tyrosine + NADPH + CO2
-
 ?
1.3.1.78 additional information TyrAAT1 is four times more efficient in catalyzing arogenate dehydrogenase reaction than TyrAAT2. TyrAAT2 presents a weak prephenate dehydrogenase activity whereas Tyr-AAT1 does not Arabidopsis thaliana ?
-
 ?
1.3.1.78 prephenate + NADP+
-
 Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + CO2 + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.78 monomer 1 * 66000, recombinant TyrAAT1, SDS-PAGE Arabidopsis thaliana
1.3.1.78 oligomer recombinant TyrAAT2 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.3.1.78 arogenate dehydrogenase isoform 1
-
 Arabidopsis thaliana
1.3.1.78 arogenate dehydrogenase isoform 2
-
 Arabidopsis thaliana
1.3.1.78 TyrAAT
-
 Arabidopsis thaliana
1.3.1.78 TyrAAT1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.78 3.4
-
 prephenate
-
 Arabidopsis thaliana
1.3.1.78 37.3
-
 L-arogenate pH 7.5, 25°C, TyrAAT2 Arabidopsis thaliana
1.3.1.78 84.2
-
 arogenate pH 7.5, 25°C, TyrAAT1 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.78 NADP+
-
 Arabidopsis thaliana
1.3.1.78 NADPH
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.78 0.0075
-
 L-tyrosine pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 0.0081
-
 L-tyrosine pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 0.0142
-
 L-tyrosine pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 0.0166
-
 L-tyrosine pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 0.0539
-
 NADPH pH 7.5, 25°C, with 0.02 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 0.0588
-
 NADPH pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 2.4
-
 prephenate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 4.2
-
 prephenate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 5.8
-
 cis-aconitate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 17.8
-
 AMP pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 20.2
-
 prephenate pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 22.5
-
 prephenate pH 7.5, 25°C, with 0.07 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 25.3
-
 cis-aconitate pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 25.9
-
 AMP pH 7.5, 25°C, with 0.05 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 27.7
-
 cis-aconitate pH 7.5, 25°C, with 0.1 mM NADP+ as fixed substrate and arogenate as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 42.9
-
 cis-aconitate pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana
1.3.1.78 73.9
-
 AMP pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT2 Arabidopsis thaliana
1.3.1.78 115
-
 AMP pH 7.5, 25°C, with 0.2 mM arogenate as fixed substrate and NADP+ as varied substrate, TyrAAT1 Arabidopsis thaliana