EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.1 | aquacobalamin | stimulates the activity of the purified H2: heterodisulfide oxidoreductase complex | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.98.1 | 0.03 | - |
H2 | pH 7, 65°C, H2: heterodisulfide oxidoreductase complex | Methanothermobacter thermautotrophicus | |
1.8.98.1 | 0.2 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine | pH 7, 65°C, H2: heterodisulfide oxidoreductase complex | Methanothermobacter thermautotrophicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.98.1 | cytoplasmic membrane | loosely associated with | Methanothermobacter thermautotrophicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.1 | Iron | 1 mol H2: heterodisulfide oxidoreductase complex with MW 250000 Da contains approximately 26 mol non-heme iron | Methanothermobacter thermautotrophicus | |
1.8.98.1 | Ni | 1 mol H2: heterodisulfide oxidoreductase complex with MW 250000 Da contains approximately 0.6 mol nickel | Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.98.1 | 250000 | - |
hexameric complex, H2:heterodisulfide oxidoreductase complex consisting of F420-non-reducing hydrogenase and heterodisulfide reductase, 20% is eluted as a dodecameric complex with MW 250000 Da, gel filtration | Methanothermobacter thermautotrophicus |
1.8.98.1 | 550000 | - |
dodecameric complex, H2:heterodisulfide oxidoreductase complex consisting of F420-non-reducing hydrogenase and heterodisulfide reductase, 20% is eluted as a hexameric complex with MW 250000 Da, gel filtration | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | Methanothermobacter thermautotrophicus | energy-conserving step | coenzyme B + coenzyme M | - |
? | |
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | energy-conserving step | coenzyme B + coenzyme M | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.98.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
1.8.98.1 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.98.1 | H2:heterodisulfide oxidoreductase complex | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M | - |
? | |
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | energy-conserving step | Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M | - |
? | |
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | coenzyme B + coenzyme M | - |
? | |
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | energy-conserving step | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | coenzyme B + coenzyme M | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.98.1 | More | H2:heterodisulfide oxidoreductase complex consisting of F420-non-reducing hydrogenase and heterodisulfide reductase is composed of six different subunits of MW 80000 Da, 51000 Da, 41000 Da, 36000 Da, 21000 Da and 17000 Da. The complex partially dissociates into two subcomplexes. The first subcomplex is composed of the 51000 Da, 41000 Da and 17000 Da subunits. The other subcomplex is composed of the 80000 Da, 36000 Da and 21000 Da subunits | Methanothermobacter thermautotrophicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.98.1 | More | H2:heterodisulfide oxidoreductase complex consists of F420-non-reducing hydrogenase and heterodisulfide reductase | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.98.1 | 7 | - |
H2:heterodisulfide oxidoreductase activity | Methanothermobacter thermautotrophicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.1 | FAD | 1 mol H2: heterodisulfide oxidoreductase complex with MW 250000 Da contains approximately 0.9 mol FAD | Methanothermobacter thermautotrophicus | |
1.8.98.1 | additional information | no reaction with conenzyme F420 | Methanothermobacter thermautotrophicus |