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Literature summary extracted from

  • Setzke, E.; Hedderich, R.; Heiden, S.; Thauer, R.K.
    H2:heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties (1994), Eur. J. Biochem., 220, 139-148.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.98.1 aquacobalamin stimulates the activity of the purified H2: heterodisulfide oxidoreductase complex Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.98.1 0.03
-
H2 pH 7, 65°C, H2: heterodisulfide oxidoreductase complex Methanothermobacter thermautotrophicus
1.8.98.1 0.2
-
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine pH 7, 65°C, H2: heterodisulfide oxidoreductase complex Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.98.1 cytoplasmic membrane loosely associated with Methanothermobacter thermautotrophicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.98.1 Iron 1 mol H2: heterodisulfide oxidoreductase complex with MW 250000 Da contains approximately 26 mol non-heme iron Methanothermobacter thermautotrophicus
1.8.98.1 Ni 1 mol H2: heterodisulfide oxidoreductase complex with MW 250000 Da contains approximately 0.6 mol nickel Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.98.1 250000
-
hexameric complex, H2:heterodisulfide oxidoreductase complex consisting of F420-non-reducing hydrogenase and heterodisulfide reductase, 20% is eluted as a dodecameric complex with MW 250000 Da, gel filtration Methanothermobacter thermautotrophicus
1.8.98.1 550000
-
dodecameric complex, H2:heterodisulfide oxidoreductase complex consisting of F420-non-reducing hydrogenase and heterodisulfide reductase, 20% is eluted as a hexameric complex with MW 250000 Da, gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 Methanothermobacter thermautotrophicus energy-conserving step coenzyme B + coenzyme M
-
?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 Methanothermobacter thermautotrophicus Marburg / DSM 2133 energy-conserving step coenzyme B + coenzyme M
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.98.1 Methanothermobacter thermautotrophicus
-
-
-
1.8.98.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.1 H2:heterodisulfide oxidoreductase complex Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2
-
Methanothermobacter thermautotrophicus coenzyme B + coenzyme M
-
?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step Methanothermobacter thermautotrophicus coenzyme B + coenzyme M
-
?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 coenzyme B + coenzyme M
-
?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step Methanothermobacter thermautotrophicus Marburg / DSM 2133 coenzyme B + coenzyme M
-
?

Subunits

EC Number Subunits Comment Organism
1.8.98.1 More H2:heterodisulfide oxidoreductase complex consisting of F420-non-reducing hydrogenase and heterodisulfide reductase is composed of six different subunits of MW 80000 Da, 51000 Da, 41000 Da, 36000 Da, 21000 Da and 17000 Da. The complex partially dissociates into two subcomplexes. The first subcomplex is composed of the 51000 Da, 41000 Da and 17000 Da subunits. The other subcomplex is composed of the 80000 Da, 36000 Da and 21000 Da subunits Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.8.98.1 More H2:heterodisulfide oxidoreductase complex consists of F420-non-reducing hydrogenase and heterodisulfide reductase Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.98.1 7
-
H2:heterodisulfide oxidoreductase activity Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.98.1 FAD 1 mol H2: heterodisulfide oxidoreductase complex with MW 250000 Da contains approximately 0.9 mol FAD Methanothermobacter thermautotrophicus
1.8.98.1 additional information no reaction with conenzyme F420 Methanothermobacter thermautotrophicus