Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roy, I.; Gupta, M.N.
    Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads (2004), Enzyme Microb. Technol., 34, 26-32.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.3 biotechnology immobilization of the enzyme on alginate beads for large-scale hydrolysis of starch in a fluidized bed of enzyme-alginate particles, method development, comparison to packed and batch mode, overview Aspergillus niger
3.2.1.41 biotechnology use of enzyme entrapped in calcium alginate beads for hydrolysis of starch Bacillus acidopullulyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger
-
-
-
3.2.1.41 Bacillus acidopullulyticus
-
 commercial preparation Promozyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 commercial preparation purified enzyme Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O soluble potato starch Aspergillus niger starch + beta-D-glucose
-
 ?
3.2.1.41 starch + H2O together with amylase, both enzymes immobilized on calcium alginate beads Bacillus acidopullulyticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 65
-
 assay at Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 55
-
 120 min, no residual activity of free enzyme, 50% residual activity of enzyme immobilized on calcium alginate beads Bacillus acidopullulyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
 assay at Aspergillus niger