Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ryan, S.; Schnitzhofer, W.; Tzanov, T.; Cavaco-Paulo, A.; Gubitz, G.M.
    An acid-stable laccase from Sclerotium rolfsii with potential for wool dye decolorization (2003), Enzyme Microb. Technol., 33, 766-774.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.2 degradation use of enzyme to decolourize textile dye Athelia rolfsii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 diethyldithiocarbamate 50% inhibition at 1.2 mM Athelia rolfsii
1.10.3.2 EDTA 50% inhibition at about 300 mM Athelia rolfsii
1.10.3.2 ethanol 50% inhibition at 36% Athelia rolfsii
1.10.3.2 L-cysteine 50% inhibition at 0.52 mM Athelia rolfsii
1.10.3.2 methanol 50% inhibition at 37% Athelia rolfsii
1.10.3.2 Sodium azide 50% inhibition at 0.009 mM Athelia rolfsii
1.10.3.2 sodium bromide 50% inhibition at 195 mM Athelia rolfsii
1.10.3.2 sodium chloride 50% inhibition at 52 mM Athelia rolfsii
1.10.3.2 Sodium fluoride 50% inhibition at 0.08 mM Athelia rolfsii
1.10.3.2 thiamine 50% inhibition at 0.95 mM Athelia rolfsii
1.10.3.2 thioglycolic acid 50% inhibition at 0.5 mM Athelia rolfsii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0168
-
Diamond Black PV 200 25°C, pH 4.5 Athelia rolfsii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 59000
-
x * 59000, SDS-PAGE Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Athelia rolfsii
-
isozymes SRL1, induced by 2,5-xylidine with culture medium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 isozyme SRL1 Athelia rolfsii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 sclerotium
-
Athelia rolfsii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 14.7
-
25°C, pH 4.5 Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 82% of activity with syringaldazine Athelia rolfsii ? + H2O
-
?
1.10.3.2 2,6-dimethoxyphenol + O2 60% of activity with syringaldazine Athelia rolfsii ? + H2O
-
?
1.10.3.2 catechol + O2 11% of activity with syringaldazine Athelia rolfsii ? + H2O
-
?
1.10.3.2 Diamond Black PV 200 + ? i.e. Mordant Black 9, Eriochrome Black PV, wool azo dye Athelia rolfsii 2-amino-1-phenol-4-sulfonic acid + 1,5-naphthalenediol + ?
-
?
1.10.3.2 guaiacol + O2 21% of activity with syringaldazine Athelia rolfsii ? + H2O
-
?
1.10.3.2 Indigo + ? textile dye Athelia rolfsii ?
-
?
1.10.3.2 Lancet Marine Blue + ? textile dye Athelia rolfsii ?
-
?
1.10.3.2 lignin sulfonic acid + O2 0.9% of activity with syringaldazine Athelia rolfsii ? + H2O
-
?
1.10.3.2 pyrogallol + O2 7% of activity with syringaldazine Athelia rolfsii ? + H2O
-
?
1.10.3.2 Remazol Brilliand Red + ? textile dye Athelia rolfsii ?
-
?
1.10.3.2 syringaldazine + O2
-
Athelia rolfsii ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 59000, SDS-PAGE Athelia rolfsii

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 SRL1
-
Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 62
-
-
Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
pH 4.5, 60 min, 50% residual activity Athelia rolfsii
1.10.3.2 80
-
pH 4.5, less than 2 min, 50% residual activity Athelia rolfsii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 80.4
-
Diamond Black PV 200 25°C, pH 4.5 Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.4
-
-
Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2.5
-
30°C, 8 min, 50% residual activity Athelia rolfsii
1.10.3.2 3
-
30°C, 12 min, 50% residual activity Athelia rolfsii
1.10.3.2 4
-
18°C, 1 week, 50% residual activity Athelia rolfsii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Athelia rolfsii isoelectric focusing
-
4.9