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Literature summary extracted from

  • Krauss, F.; Guenther, H.; Skopan, H.; Simon, H.
    Additional experiences with Proteus vulgaris and its hydroxycarboxylate-oxidoreductase for the preparation of (2R)- or (2S)-hydroxycarboxylates (1988), DECHEMA Biotechnol. Conf., 1, 313-318.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.30 tungstate complete inhibition in vivo Proteus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.30 Iron-sulfur cluster
-
Proteus vulgaris
1.1.99.30 molybdate strong induction Proteus vulgaris
1.1.99.30 Molybdenum 4-12 atoms per enzyme molecule Proteus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.30 750000
-
-
Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.30 Proteus vulgaris
-
molybdate-inducible
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.30 60fold Proteus vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.30 810
-
crude cell extract Proteus vulgaris

Storage Stability

EC Number Storage Stability Organism
1.1.99.30 -16°C, N2-atmosphere, wet packed cells, 450 days, more than 90% remaining activity Proteus vulgaris
1.1.99.30 room temperature, freeze dried cells, 200 days, more than 80% remaining activity Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.30 (2R)-hydroxy-4-methylpentanoate + 2-oxo-butyrate
-
Proteus vulgaris 2-oxo-4-methylpentanoate + (2R)-hydroxybutyrate
-
r
1.1.99.30 2-hydroxyglutarate + oxidized acceptor
-
Proteus vulgaris 2-oxoglutarate + reduced acceptor
-
r
1.1.99.30 2-oxo-3,5-dienoates + H2
-
Proteus vulgaris ?
-
r
1.1.99.30 2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2
-
Proteus vulgaris rosmarinic acid
-
r
1.1.99.30 2-oxo-3-enoates + H2
-
Proteus vulgaris ?
-
r
1.1.99.30 lactate + oxidized acceptor
-
Proteus vulgaris pyruvate + reduced acceptor
-
r
1.1.99.30 mandelate + oxidized acceptor
-
Proteus vulgaris oxo(phenyl)acetic acid + reduced acceptor
-
r
1.1.99.30 pantolactone + oxidized acceptor
-
Proteus vulgaris dehydropantolactone + reduced acceptor
-
r
1.1.99.30 phenylpyruvate + H2
-
Proteus vulgaris (2R)-phenyllactate
-
r
1.1.99.30 phenylpyruvate + reduced benzyl viologen
-
Proteus vulgaris (2R)-phenyllactate + oxidized benzyl viologen
-
r
1.1.99.30 phenylpyruvate + reduced carbamoylmethyl viologen
-
Proteus vulgaris (2R)-phenyllactate + oxidized carbamoylmethyl viologen
-
r
1.1.99.30 phenylpyruvate + reduced cobalt sepulchrate
-
Proteus vulgaris (2R)-phenyllactate + oxidized cobalt sepulchrate
-
r
1.1.99.30 phenylpyruvate + reduced indigocarmine
-
Proteus vulgaris (2R)-phenyllactate + oxidized indigocarmine
-
r
1.1.99.30 phenylpyruvate + reduced kresyl violet
-
Proteus vulgaris (2R)-phenyllactate + oxidized kresyl violet
-
r
1.1.99.30 phenylpyruvate + reduced meldolablue
-
Proteus vulgaris (2R)-phenyllactate + oxidized meldolablue
-
r
1.1.99.30 phenylpyruvate + reduced methylene blue
-
Proteus vulgaris (2R)-phenyllactate + oxidized methylene blue
-
r
1.1.99.30 phenylpyruvate + reduced phenosafranine
-
Proteus vulgaris (2R)-phenyllactate + oxidized phenosafranine
-
r
1.1.99.30 phenylpyruvate + reduced safranine TH
-
Proteus vulgaris (2R)-phenyllactate + oxidized safranine TH
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.99.30 HVOR
-
Proteus vulgaris
1.1.99.30 hydroxycarboxlate-oxidoreductase
-
Proteus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.30 35
-
assay at Proteus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.30 7
-
assay at Proteus vulgaris