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Literature summary extracted from

  • Healy, F.G.; Latorre, C.; Albrecht, S.L.; Reddy, P.M.; Shanmugam, K.T.
    Altered kinetic properties of tyrosine-183 to cysteine mutation in glutamine synthetase of Anabaena variabilis strain SA1 is responsible for excretion of ammonium ion produced by nitrogenase (2003), Curr. Microbiol., 46, 423-431.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 SA0 and SA1 expressed in Escherichia coli SE3000 Trichormus variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 alanine more inhibitory for enzyme from strain SA0 than for enzyme from SA1 Trichormus variabilis
6.3.1.2 aspartate more inhibitory for enzyme from strain SA0 than for enzyme from SA1 Trichormus variabilis
6.3.1.2 cysteine more inhibitory for enzyme from strain SA0 than for enzyme from SA1 Trichormus variabilis
6.3.1.2 glycine more inhibitory for enzyme from strain SA0 than for enzyme from SA1 Trichormus variabilis
6.3.1.2 L-methionine sulfone inhibits enzyme from strain SA0 but not from SA1, more than 80% inhibition at 0.05 mM Trichormus variabilis
6.3.1.2 L-methionine-DL-sulfoximine inhibits enzyme from strain SA0 but not from SA1, more than 80% inhibition at 0.05 mM Trichormus variabilis
6.3.1.2 Mn2+ inhibitory for enzyme from strain SA0 Trichormus variabilis
6.3.1.2 serine more inhibitory for enzyme from strain SA0 than for enzyme from SA1 Trichormus variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.004
-
 NH4+ enzyme from strain SA0, pH 6.8 Trichormus variabilis
6.3.1.2 0.015
-
 NH4+ enzyme from strain SA0, pH 7.5 Trichormus variabilis
6.3.1.2 0.1
-
 ATP enzyme from strain SA0, pH 6.8 Trichormus variabilis
6.3.1.2 0.13
-
 ATP enzyme from strain SA0, pH 7.5 Trichormus variabilis
6.3.1.2 0.17
-
 ATP enzyme from strain SA1, pH 6.8 Trichormus variabilis
6.3.1.2 0.21
-
 ATP enzyme from strain SA1, pH 7.5 Trichormus variabilis
6.3.1.2 1.4
-
 glutamate enzyme from strain SA0, pH 6.8 Trichormus variabilis
6.3.1.2 1.4
-
 NH4+ enzyme from strain SA1, pH 7.5 Trichormus variabilis
6.3.1.2 1.7
-
 glutamate enzyme from strain SA0, pH 7.5 Trichormus variabilis
6.3.1.2 9.2
-
 glutamate enzyme from strain SA1, pH 6.8 Trichormus variabilis
6.3.1.2 12.4
-
 NH4+ enzyme from strain SA1, pH 6.8 Trichormus variabilis
6.3.1.2 16.8
-
 glutamate enzyme from strain SA1, pH 7.5 Trichormus variabilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ required for optimum activity of enzyme from strain SA0, no activation of enzyme from strain SA1 Trichormus variabilis
6.3.1.2 Mn2+ required for optimum activity of enzyme from strain SA1, highest activity at 0.3 mM, concentration inhibitory for enzyme from SA0 Trichormus variabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 52000
-
 SDS-PAGE Trichormus variabilis
6.3.1.2 53080
-
 calculated from amino acid sequence Trichormus variabilis
6.3.1.2 625000
-
 gel filtration Trichormus variabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + glutamate + NH4+ Trichormus variabilis central enzyme of nitrogen metabolism, provides glutamine for biosynthesis, ammonia assimilation ADP + phosphate + L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Trichormus variabilis
-
 strains SA0 and SA1, SA1 carries a single amino acid mutation Y183C and is defective in ammonia assimilation below concentrations of 0.5 mM
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 from both strains Trichormus variabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 12.1
-
 purified enzyme from strain SA1 Trichormus variabilis
6.3.1.2 126.1
-
 purified enzyme from strain SA0 Trichormus variabilis

Storage Stability

EC Number Storage Stability Organism
6.3.1.2 -70°C, concentrated solution in 50% glycerol, 2 months, 4% loss of activity Trichormus variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + glutamate + NH4+
-
 Trichormus variabilis ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, provides glutamine for biosynthesis, ammonia assimilation Trichormus variabilis ADP + phosphate + L-glutamine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer 12 * 52000, subunit mass by SDS-PAGE, native mass by gel filtration Trichormus variabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.8
-
 strain SA1 Trichormus variabilis
6.3.1.2 7.5
-
 strain SA0 Trichormus variabilis