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Literature summary extracted from

  • Sarower, M.G.; Okada, S.; Abe, H.
    Catalytic and structural characteristics of carp hepatopancreas D-amino acid oxidase expressed in Escherichia coli (2005), Comp. Biochem. Physiol. B, 140, 417-425.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 overexpression in Escherichia coli Cyprinus carpio

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 benzoate 75 mM, 76% inhibition Cyprinus carpio
1.4.3.3 creatinine 75 mM, 86% inhibition Cyprinus carpio
1.4.3.3 HgCl2 0.1 mM, 71% inhibition Cyprinus carpio
1.4.3.3 PCMB 75 mM, 93% inhibition Cyprinus carpio
1.4.3.3 Silver nitrate 0.1 mM, 66% inhibition Cyprinus carpio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.226
-
 D-Tyr pH 8.0, 37°C Cyprinus carpio
1.4.3.3 0.23
-
 D-Ala pH 8.0, 37°C Cyprinus carpio
1.4.3.3 0.263
-
 D-Val pH 8.0, 37°C Cyprinus carpio
1.4.3.3 0.357
-
 D-Phe pH 8.0, 37°C Cyprinus carpio
1.4.3.3 0.425
-
 D-Lys pH 8.0, 37°C Cyprinus carpio
1.4.3.3 0.909
-
 D-Ser pH 8.0, 37°C Cyprinus carpio
1.4.3.3 1.06
-
 D-Ile pH 8.0, 37°C Cyprinus carpio
1.4.3.3 1.13
-
 D-Pro pH 8.0, 37°C Cyprinus carpio
1.4.3.3 6.5
-
 D-Leu pH 8.0, 37°C Cyprinus carpio

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Cyprinus carpio Q6TGN2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 recombinant enzyme Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 hepatopancreas
-
 Cyprinus carpio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 293
-
-
 Cyprinus carpio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-Ala + H2O + O2
-
 Cyprinus carpio pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ile + H2O + O2
-
 Cyprinus carpio 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Leu + H2O + O2
-
 Cyprinus carpio 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Lys + H2O + O2
-
 Cyprinus carpio ? + NH3 + H2O2
-
 ?
1.4.3.3 D-Phe + H2O + O2
-
 Cyprinus carpio phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Pro + H2O + O2
-
 Cyprinus carpio 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2
-
 Cyprinus carpio 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 D-Tyr + H2O + O2
-
 Cyprinus carpio 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Val + H2O + O2
-
 Cyprinus carpio alpha-ketoisovalerate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 chDAO
-
 Cyprinus carpio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 35
-
-
 Cyprinus carpio

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.3 20 60 20°C: about 75% of maximal activity, 60°C: about 40% of maximal activity Cyprinus carpio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 50
-
 stable below Cyprinus carpio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 34.5
-
 D-Ser pH 8.0, 37°C Cyprinus carpio
1.4.3.3 42.3
-
 D-Lys pH 8.0, 37°C Cyprinus carpio
1.4.3.3 76.7
-
 D-Ile pH 8.0, 37°C Cyprinus carpio
1.4.3.3 116
-
 D-Leu pH 8.0, 37°C Cyprinus carpio
1.4.3.3 117
-
 D-Tyr pH 8.0, 37°C Cyprinus carpio
1.4.3.3 119
-
 D-Phe pH 8.0, 37°C Cyprinus carpio
1.4.3.3 142
-
 D-Pro pH 8.0, 37°C Cyprinus carpio
1.4.3.3 170
-
 D-Val pH 8.0, 37°C Cyprinus carpio
1.4.3.3 190
-
 D-Ala pH 8.0, 37°C Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8.5
-
-
 Cyprinus carpio

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.3 6.5 11 pH 6.5: about 75% of maximal activity, pH 11.0: about 75% of maximal activity Cyprinus carpio

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 5.5 11 1 h, stable Cyprinus carpio

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.3 5.1
-
 creatinine pH 8.0, 37°C Cyprinus carpio
1.4.3.3 6
-
 PCMB pH 8.0, 37°C Cyprinus carpio
1.4.3.3 12.5
-
 benzoate pH 8.0, 37°C Cyprinus carpio