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Literature summary extracted from

  • Kan-no, N.; Sato, M.; Yokoyama, T.; Nagahisa, E.
    Occurrence of beta-alanine-specific opine dehydrogenase in the muscle of the limpet Cellana grata Gould (Archaeogastropoda) (1999), Comp. Biochem. Physiol. B, 123B, 125-136.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.26 0.032
-
 NADH pH 7.1, 30°C Cellana grata
1.5.1.26 0.078
-
 NAD+ pH 9.0, 30°C Cellana grata
1.5.1.26 0.19
-
 pyruvate pH 7.1, 30°C Cellana grata
1.5.1.26 14.3
-
 beta-Alanine pH 7.1, 30°C Cellana grata
1.5.1.26 35.2
-
 beta-alanopine pH 9.0, 30°C Cellana grata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.26 39700
-
 gel filtration Cellana grata
1.5.1.26 40800
-
 x * 40800, SDS-PAGE Cellana grata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.26 beta-alanine + pyruvate + NADH Cellana grata specific for beta-alanine beta-alanopine + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.26 Cellana grata
-
 Gold, isozyme beta-AlDH-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.26 native enzyme by ammonium sulfate fractionation and several chromatographic steps, 705.6fold Cellana grata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.26 muscle
-
 Cellana grata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.26 additional information
-
-
 Cellana grata
1.5.1.26 13
-
 purified enzyme, reverse reaction Cellana grata
1.5.1.26 190.5
-
 purified enzyme, forward reaction Cellana grata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.26 beta-alanine + pyruvate + NADH specific for beta-alanine Cellana grata beta-alanopine + NAD+ + H2O
-
 ?
1.5.1.26 additional information tauropine, alanopine, strombine, and octopine are poor substrates Cellana grata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.26 monomer x * 40800, SDS-PAGE Cellana grata

Synonyms

EC Number Synonyms Comment Organism
1.5.1.26 beta-AlDH-2
-
 Cellana grata
1.5.1.26 More the enzyme belongs to the opine dehydrogenase family of enzymes Cellana grata
1.5.1.26 OpDH
-
 Cellana grata
1.5.1.26 opine dehydrogenase
-
 Cellana grata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.26 30
-
 assay at Cellana grata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.26 7.1
-
 opine biosynthetic reaction direction Cellana grata
1.5.1.26 9
-
 catabolic reaction direction Cellana grata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.26 5 10 half-maximal forward reaction at pH 5.8, half-maximal reverse reaction at pH 8.5 Cellana grata

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.26 NAD+
-
 Cellana grata
1.5.1.26 NADH
-
 Cellana grata