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Literature summary extracted from

  • Stroemberg, P.; Svensson, S.; Hedberg, J.J.; Nordling, E.; Hoog, J.O.
    Identification and characterisation of two allelic forms of human alcohol dehydrogenase 2 (2002), Cell. Mol. Life Sci., 59, 552-559.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 5-7 genes encoding ADH, DNA and amino acid sequence determination and analysis, polymorphism and allelic frequencies analysis, gene ADH2 possesses 2 allelic forms with Ile308 or Val308, expression of ADH2 alloenzymes in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 additional information
-
 additional information steady-state kinetics, MW 25 kDa Homo sapiens
1.1.1.1 0.009
-
 Octanol recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.011
-
 retinol recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.012
-
 retinol recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.016
-
 Octanol recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.063
-
 NAD+ recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.074
-
 NAD+ recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens
1.1.1.1 9
-
 ethanol recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 10.6
-
 ethanol recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Homo sapiens
-
 alcohol dehydrogenase 2, 2 allelic forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 recombinant ADH2 alloenzymes from Escherichia coli by DEAE and AMP or blue Sepharose chromatography, and ultrafiltration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 ethanol + NAD+
-
 Homo sapiens acetaldehyde + NADH
-
 ?
1.1.1.1 octanol + NAD+
-
 Homo sapiens octanal + NADH
-
 ?
1.1.1.1 retinol + NAD+
-
 Homo sapiens retinal + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 More structure modelling Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 25
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 additional information
-
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.038
-
 Octanol recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.04
-
 Octanol recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.087
-
 retinol recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.11
-
 retinol recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.167
-
 ethanol recombinant allozyme Ile308, pH 7.5, 25°C Homo sapiens
1.1.1.1 0.175
-
 ethanol recombinant allozyme Val308, pH 7.5, 25°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 7.5
-
 kinetic analysis assay at Homo sapiens
1.1.1.1 10
-
 standard assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Homo sapiens