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Literature summary extracted from

  • Kuroyama, H.; Tsutsui, N.; Hashimoto, Y.; Tsumuraya, Y.
    Purification and characterization of a beta-glucuronidase from Aspergillus niger (2001), Carbohydr. Res., 333, 27-39.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 D-glucaric acid 1,4-lactone 5 mM, 30% inhibition Aspergillus niger
3.2.1.31 Hg2+ 1 mM, 37°C, 10 min, 23% inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.23
-
 p-nitrophenyl-beta-D-glucuronide pH 4.6, 37°C Aspergillus niger
3.2.1.31 0.53
-
 beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal pH 4.6, 37°C Aspergillus niger
3.2.1.31 0.71
-
 beta-GlcA-(1-6)-Gal pH 4.6, 37°C Aspergillus niger
3.2.1.31 1.4
-
 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal pH 4.6, 37°C Aspergillus niger
3.2.1.31 1.9
-
 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal pH 4.6, 37°C Aspergillus niger
3.2.1.31 3.6
-
 4-Me-beta-GlcA-(1-6)-Gal pH 4.6, 37°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 68000
-
 1 * 68000, SDS-PAGE Aspergillus niger
3.2.1.31 71000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31
-
 Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.31 commercial preparation Pectinex Ultra SP-L, Lot No. G 770 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 22.6
-
 reaction with p-nitrophenyl-beta-D-glucuronide Aspergillus niger
3.2.1.31 29.3
-
 reaction with beta-GlcA-(1-6)-Gal Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
3.2.1.31 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
3.2.1.31 4-Me-beta-GlcA-(1-6)-Gal + H2O rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
3.2.1.31 alpha-L-arabinosidase-treated-arabinogalactan-protein + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.31 beta-GlcA-(1-3)-Gal + H2O rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
3.2.1.31 beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger beta-Gal-(1-6)-Gal + beta-D-glucuronic acid
-
 ?
3.2.1.31 beta-GlcA-(1-6)-Gal + H2O rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger D-galactose + beta-D-glucuronic acid
-
 ?
3.2.1.31 additional information transglycosylation activity Aspergillus niger ?
-
 ?
3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-glucuronic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 monomer 1 * 68000, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 60
-
 pH 4.6, 20 min, stable Aspergillus niger
3.2.1.31 75
-
 pH 4.6, 20 min, complete inactivation Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 3
-
 reaction with beta-GlcA-(1-6)-Gal and p-nitrophenyl-beta-D-glucuronide Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.31 2 9 4°C, 24 h, stable Aspergillus niger
3.2.1.31 4.5 9 37°C, 3 h, stable Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.31 Aspergillus niger isoelectric focusing, pH-range: 3.5-10
-
 5