Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, C.; Liu, Q.; Song, X.; Ding, D.; Ji, A.; Qu, Y.
    Epoxide hydrolase-catalyzed resolution of ethyl 3-phenylglycidate using whole cells of Pseudomonas sp (2003), Biotechnol. Lett., 25, 2113-2116.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.3.2.9 additional information maximal activity with dimethyl formamide as co-solvent, effects of solvents on enzyme activity, overview Pseudomonas sp.
3.3.2.10 additional information maximal activity with dimethyl formamide as co-solvent, effects of solvents on enzyme activity, overview Pseudomonas sp.

Application

EC Number Application Comment Organism
3.3.2.9 synthesis the enantioselective enzyme is useful in production of chiral substances, e.g. production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat by whole cells of Pseudomonas sp. strain BZS21, maximal activity with dimethyl formamide as co-solvent Pseudomonas sp.
3.3.2.10 synthesis the enantioselective enzyme is useful in production of chiral substances, e.g. production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat by whole cells of Pseudomonas sp. strain BZS21, maximal activity with dimethyl formamide as co-solvent Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.9 Pseudomonas sp.
-
-
-
3.3.2.9 Pseudomonas sp. BZS21
-
-
-
3.3.2.10 Pseudomonas sp.
-
-
-
3.3.2.10 Pseudomonas sp. BZS21
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.9 culture condition:styrene oxide-grown cell
-
 Pseudomonas sp.
-
3.3.2.9 culture condition:styrene-grown cell
-
 Pseudomonas sp.
-
3.3.2.10 culture condition:styrene oxide-grown cell
-
 Pseudomonas sp.
-
3.3.2.10 culture condition:styrene-grown cell
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.9 racemic ethyl 3-phenylglycidate + H2O
-
 Pseudomonas sp. (2R,3S)-ethyl 3-phenylglycidate production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat ?
3.3.2.9 racemic ethyl 3-phenylglycidate + H2O
-
 Pseudomonas sp. BZS21 (2R,3S)-ethyl 3-phenylglycidate production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat ?
3.3.2.10 racemic ethyl 3-phenylglycidate + H2O production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat Pseudomonas sp. ?
-
 ?
3.3.2.10 racemic ethyl 3-phenylglycidate + H2O production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat Pseudomonas sp. BZS21 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.9 30
-
 assay at Pseudomonas sp.
3.3.2.10 30
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.9 8
-
 assay at Pseudomonas sp.
3.3.2.10 8
-
 assay at Pseudomonas sp.