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Literature summary extracted from

  • Rhee, C.H.; Kim, K.P.; Park, H.D.
    Two novel extracellular cholesterol oxidases of Bacillus sp. isolated from fermented flatfish (2002), Biotechnol. Lett., 24, 1385-1389.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 4.5
-
 cholesterol pH 7, 30°C, enzyme CO2 Bacillus sp. (in: Bacteria)
1.1.3.6 6.76
-
 cholesterol pH 7, 30°C, enzyme CO1 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.6 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 36000
-
 1 * 36000, enzyme CO1, SDS-PAGE Bacillus sp. (in: Bacteria)
1.1.3.6 37000
-
 1 * 37000, enzyme CO2, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Bacillus sp. (in: Bacteria)
-
 SFF34
-
1.1.3.6 Bacillus sp. (in: Bacteria) SFF34
-
 SFF34
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 7.6
-
 enzyme CO1 Bacillus sp. (in: Bacteria)
1.1.3.6 8
-
 enzyme CO2 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 1,4-androstadiene-3,17-dione + H2O 11% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 5alpha-cholestane + O2 72% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 5alpha-cholestane + O2 90% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 5alpha-cholestane-3beta-ol-7-one + O2 74% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) 5alpha-cholestane-3,7,dione
-
 ?
1.1.3.6 5alpha-cholestane-3beta-ol-7-one + O2 84% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) 5alpha-cholestane-3,7,dione
-
 ?
1.1.3.6 beta-sitosterol + O2 57% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 79% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 57% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) SFF34 stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 79% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) SFF34 stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Bacillus sp. (in: Bacteria) cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Bacillus sp. (in: Bacteria) SFF34 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol-5alpha,6beta-epoxide + O2 10% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 cholesterol-5alpha,6beta-epoxide + O2 9% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 coprostane + O2 63% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 coprostane + O2 67% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) ?
-
 ?
1.1.3.6 dihydrocholesterol + O2 58% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 dihydrocholesterol + O2 70% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 hecogenin + O2 66% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) 5alpha-(25R)-spirostan-3,12-dione + H2O2
-
 ?
1.1.3.6 hecogenin + O2 80% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) 5alpha-(25R)-spirostan-3,12-dione + H2O2
-
 ?
1.1.3.6 lanosterol + O2 4% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) 5alpha-lanosta-8,24-dien-3-one + H2O2
-
 ?
1.1.3.6 lanosterol + O2 5% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) 5alpha-lanosta-8,24-dien-3-one + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 102% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) stigmasta-5,22-dien-3-one + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 86% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) stigmasta-5,22-dien-3-one + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 102% of the activity with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria) SFF34 stigmasta-5,22-dien-3-one + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 86% of the activity with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria) SFF34 stigmasta-5,22-dien-3-one + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.6 monomer 1 * 36000, enzyme CO1, SDS-PAGE Bacillus sp. (in: Bacteria)
1.1.3.6 monomer 1 * 37000, enzyme CO2, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 CO1
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 40
-
 reaction with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria)
1.1.3.6 60
-
 reaction with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 30 55 30°C: about 65% of maximal activity, 55°C: about 60% of maximal activity, reaction with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria)
1.1.3.6 35 75 about 50% of maximal activity at 35°C and at 75°C, reaction with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 60
-
 1 h, about 10% loss of activity, enzyme CO1 Bacillus sp. (in: Bacteria)
1.1.3.6 60
-
 1 h, about 10% loss of activity, enzyme CO2 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 6
-
 reaction with cholesterol, enzyme CO2 Bacillus sp. (in: Bacteria)
1.1.3.6 6.5
-
 reaction with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 9 pH 5: 83% of maximal activity, pH 9.0: 77% of maximal activity, reaction with cholesterol, enzyme CO1 Bacillus sp. (in: Bacteria)
1.1.3.6 5 8 pH 5: 78% of maximal activity, pH 8.0: 75% of maximal activity, reaction with cholestero, enzyme CO2 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 5
-
 most stable at, enzyme CO2 Bacillus sp. (in: Bacteria)
1.1.3.6 6
-
 most stable at Bacillus sp. (in: Bacteria)