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Literature summary extracted from

  • Ait-Abdelkader, N.; De Caro, A.; Guzzo, J.; Michel, G.P.F.; Baratti, J.C.
    The intracellular sucrase (SacA) of Zymomonas mobilis is not involved in sucrose assimilation (2000), Biotechnol. Lett., 22, 461-467.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26
-
 Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 42
-
 sucrose pH 7.0, 30°C Zymomonas mobilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 intracellular
-
 Zymomonas mobilis 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 58000
-
 gel filtration Zymomonas mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.26 sucrose + H2O Zymomonas mobilis Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form beta-D-fructose + alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 recombinant enzyme Zymomonas mobilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 129
-
-
 Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 inulin + H2O 1.8% of the activity with sucrose, isoenzyme AIV I and AIV II Zymomonas mobilis beta-D-fructose + ?
-
 ?
3.2.1.26 raffinose + H2O about 30% of the activity with sucrose, isoenzyme AIV I and AIV II Zymomonas mobilis beta-D-fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Zymomonas mobilis beta-D-fructose + alpha-D-glucose
-
 ?
3.2.1.26 sucrose + H2O Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form Zymomonas mobilis beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 monomer 1 * 58000, SDS-PAGE Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 SacA
-
 Zymomonas mobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 25
-
-
 Zymomonas mobilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.26 5 30 active in the range Zymomonas mobilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 46
-
 30 min, 50% loss of activity Zymomonas mobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 125
-
 sucrose pH 7.0, 30°C Zymomonas mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6.5
-
-
 Zymomonas mobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 4.5 8.5 active in the range Zymomonas mobilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.26 Zymomonas mobilis electrofocusing
-
 7.1