Literature summary extracted from
Kosjek, B.; Stampfer, W.; Pogorevc, M.; Goessler, W.; Faber, K.; Kroutil, W.
Purification and characterization of a chemotolerant alcohol dehydrogenase applicable to coupled redox reactions (2004), Biotechnol. Bioeng., 86, 55-62.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.1.1.1 |
synthesis |
enzyme can be used in preparative scale enantioselective oxidation of sec-alcohol in asymmetric reduction of ketones, using acetone and 2-propanol, respectively, as cosubstrates for cofactor-regeneration via a coupled-substrate approach |
Rhodococcus ruber |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.1.1.1 |
Zn2+ |
1 tightly bound ion per subunit |
Rhodococcus ruber |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.1.1.1 |
38000 |
- |
2 * 38000, SDS-PAGE |
Rhodococcus ruber |
Organic Solvent Stability
EC Number |
Organic Solvent |
Comment |
Organism |
---|
1.1.1.1 |
2-propanol |
80% v/v, stable at |
Rhodococcus ruber |
1.1.1.1 |
Acetone |
50% v/v, stable at |
Rhodococcus ruber |
1.1.1.1 |
additional information |
chemotolerant enzyme |
Rhodococcus ruber |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.1 |
Rhodococcus ruber |
- |
strain DSM44541 |
- |
1.1.1.1 |
Rhodococcus ruber DSM 44541 |
- |
strain DSM44541 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.1 |
- |
Rhodococcus ruber |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.1.1.1 |
a primary alcohol + NAD+ = an aldehyde + NADH + H+ |
sequential reaction mechanism |
Rhodococcus ruber |
|
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.1.1.1 |
very stable during storage |
Rhodococcus ruber |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.1 |
alcohol + NAD+ |
medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes |
Rhodococcus ruber |
aldehyde or ketone + NADH |
- |
r |
|
1.1.1.1 |
alcohol + NAD+ |
medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes |
Rhodococcus ruber DSM 44541 |
aldehyde or ketone + NADH |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.1.1.1 |
dimer |
2 * 38000, SDS-PAGE |
Rhodococcus ruber |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.1 |
medium-chain secondary alcohol dehydrogenase |
- |
Rhodococcus ruber |
1.1.1.1 |
sec-ADH A |
- |
Rhodococcus ruber |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.1.1.1 |
30 |
50 |
- |
Rhodococcus ruber |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.1.1.1 |
50 |
- |
half-life: 35 h |
Rhodococcus ruber |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.1.1.1 |
6.5 |
- |
reduction |
Rhodococcus ruber |
1.1.1.1 |
9 |
- |
oxidation |
Rhodococcus ruber |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.1 |
NAD+ |
dependent on |
Rhodococcus ruber |
|
1.1.1.1 |
NADH |
dependent on |
Rhodococcus ruber |
|