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Literature summary extracted from

  • Kosjek, B.; Stampfer, W.; Pogorevc, M.; Goessler, W.; Faber, K.; Kroutil, W.
    Purification and characterization of a chemotolerant alcohol dehydrogenase applicable to coupled redox reactions (2004), Biotechnol. Bioeng., 86, 55-62.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.1 synthesis enzyme can be used in preparative scale enantioselective oxidation of sec-alcohol in asymmetric reduction of ketones, using acetone and 2-propanol, respectively, as cosubstrates for cofactor-regeneration via a coupled-substrate approach Rhodococcus ruber

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zn2+ 1 tightly bound ion per subunit Rhodococcus ruber

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 38000
-
2 * 38000, SDS-PAGE Rhodococcus ruber

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.1 2-propanol 80% v/v, stable at Rhodococcus ruber
1.1.1.1 Acetone 50% v/v, stable at Rhodococcus ruber
1.1.1.1 additional information chemotolerant enzyme Rhodococcus ruber

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Rhodococcus ruber
-
strain DSM44541
-
1.1.1.1 Rhodococcus ruber DSM 44541
-
strain DSM44541
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
Rhodococcus ruber

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.1 a primary alcohol + NAD+ = an aldehyde + NADH + H+ sequential reaction mechanism Rhodococcus ruber

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 very stable during storage Rhodococcus ruber

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 alcohol + NAD+ medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes Rhodococcus ruber aldehyde or ketone + NADH
-
r
1.1.1.1 alcohol + NAD+ medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes Rhodococcus ruber DSM 44541 aldehyde or ketone + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 38000, SDS-PAGE Rhodococcus ruber

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 medium-chain secondary alcohol dehydrogenase
-
Rhodococcus ruber
1.1.1.1 sec-ADH A
-
Rhodococcus ruber

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 30 50
-
Rhodococcus ruber

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 50
-
half-life: 35 h Rhodococcus ruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6.5
-
reduction Rhodococcus ruber
1.1.1.1 9
-
oxidation Rhodococcus ruber

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ dependent on Rhodococcus ruber
1.1.1.1 NADH dependent on Rhodococcus ruber