EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.8.3 | additional information | immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.8.3 | additional information | immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles, vesicle preparation overview | Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.1.5.14 | the enzyme immobilized onto giant vesicles is stable for at least 20 days at 25°C, while the activity of the free enzyme dropps to about 20% of its initial activity during the same period of time | Gluconobacter sp. |
1.5.8.3 | immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.8.3 | 1.3 | - |
sarcosine | pH 7.5, 37°C, immobilized enzyme | Pseudomonas putida | |
1.5.8.3 | 1.9 | - |
sarcosine | pH 7.5, 37°C, non-immobilized enzyme | Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.14 | membrane | - |
Gluconobacter sp. | 16020 | - |
1.5.8.3 | membrane | - |
Pseudomonas putida | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.14 | Gluconobacter sp. | - |
- |
- |
1.5.8.3 | Pseudomonas putida | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.8.3 | commercial preparation | diagnostic reagent grade | Pseudomonas putida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.8.3 | additional information | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.8.3 | storage stability at room temperature is increased for vesicle-immobilized enzyme | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.14 | D-fructose + [Fe(CN)6]3- | - |
Gluconobacter sp. | 5-dehydro-D-fructose + [Fe(CN)6]4- | - |
? | |
1.5.8.3 | sarcosine + oxidized 1-methoxy-5-methylphenazinium methylsulfate | quantification with nitroblue tetrazolium chloride, i.e. NBT or 2,2'-bis(4-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride, giving NBT-formazan in reaction with reduced 1-methoxy-5-methylphenazinium methylsulfate, overview | Pseudomonas putida | glycine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.14 | FDH | - |
Gluconobacter sp. |
1.5.8.3 | SDH | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.8.3 | 37 | - |
assay at | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.14 | 25 | - |
the enzyme immobilized onto giant vesicles is stable for at least 20 days at 25°C, while the activity of the free enzyme dropps to about 20% of its initial activity during the same period of time | Gluconobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.8.3 | 7.5 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.8.3 | additional information | 1-methoxy-5-methylphenazinium methylsulfate acts as electron acceptor | Pseudomonas putida |