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Literature summary extracted from

  • Wen, C.M.; Tseng, C.S.; Cheng, C.Y.; Li, Y.K.
    Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2 (2002), Biotechnol. Appl. Biochem., 35, 213-219.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Cu2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Hg2+
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 30000
-
 gel filtration Bacillus sp. (in: Bacteria)
3.2.1.14 36500
-
 1 * 36500, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus sp. (in: Bacteria)
-
 NCTU2
-
3.2.1.14 Bacillus sp. (in: Bacteria) NCTU2
-
 NCTU2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 312
-
 after Sephadex G-75 purification step Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 4°C, 20 mM phosphate buffer, pH 7, more than 4 months Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal Bacillus sp. (in: Bacteria) N,N'-diacetylchitobiose + ? predominant product ?
3.2.1.14 chitin + H2O colloidal Bacillus sp. (in: Bacteria) NCTU2 N,N'-diacetylchitobiose + ? predominant product ?
3.2.1.14 chitotetraose + H2O
-
 Bacillus sp. (in: Bacteria) 2 chitobiose
-
 ?
3.2.1.14 chitotetraose + H2O
-
 Bacillus sp. (in: Bacteria) NCTU2 2 chitobiose
-
 ?
3.2.1.14 N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O
-
 Bacillus sp. (in: Bacteria) 3 N,N'-diacetylchitobiose
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Bacillus sp. (in: Bacteria) N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Bacillus sp. (in: Bacteria) NCTU2 N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Bacillus sp. (in: Bacteria) N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Bacillus sp. (in: Bacteria) NCTU2 N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
3.2.1.14 partially N-acetylated chitosan + H2O variuos degrees of acetylation Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.14 partially N-acetylated chitosan + H2O variuos degrees of acetylation Bacillus sp. (in: Bacteria) NCTU2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 36500, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50 60
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 37 70
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 60
-
 stable for more than 3 h Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 5.5 9
-
 Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Bacillus sp. (in: Bacteria) isoelectric focusing
-
 6