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Literature summary extracted from

  • Kobayashi, I.; Tokuda, M.; Hashimoto, H.; Konda, T.; Nakano, H.; Kitahata, S.
    Purification and characterization of a new type of alpha-glucosidase from Paecilomyces lilacinus that has transglucosylation activity to produce alpha-1,3- and alpha-1,2-linked oligosaccharides (2003), Biosci. Biotechnol. Biochem., 67, 29-35.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.14
-
 nigerose pH 5.0, 50°C Purpureocillium lilacinum
3.2.1.20 0.16
-
 maltose pH 5.0, 50°C Purpureocillium lilacinum
3.2.1.20 0.86
-
 isomaltose pH 5.0, 50°C Purpureocillium lilacinum
3.2.1.20 1.44
-
 kojibiose pH 5.0, 50°C Purpureocillium lilacinum
3.2.1.20 2.8
-
 starch soluble substrate, pH 5.0, 50°C Purpureocillium lilacinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 intracellular
-
 Purpureocillium lilacinum 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 54000
-
 x * 54000 Purpureocillium lilacinum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Purpureocillium lilacinum
-
-
-
3.2.1.20 Purpureocillium lilacinum HKS-124
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 254fold Purpureocillium lilacinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 mycelium
-
 Purpureocillium lilacinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 12.4
-
 purified enzyme Purpureocillium lilacinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 amylose + H2O
-
 Purpureocillium lilacinum D-glucose + ?
-
 ?
3.2.1.20 D-glucose + kojibiose
-
 Purpureocillium lilacinum 4-O-alpha-kojibiosyl-D-glucose
-
 r
3.2.1.20 D-glucose + maltose transglycosylation activity Purpureocillium lilacinum maltotriose
-
 r
3.2.1.20 D-glucose + nigerose
-
 Purpureocillium lilacinum 4-O-alpha-nigerosyl-D-glucose
-
 r
3.2.1.20 glycogen + H2O low activity Purpureocillium lilacinum D-glucose + ?
-
 ?
3.2.1.20 isomaltose + H2O
-
 Purpureocillium lilacinum 2 alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Purpureocillium lilacinum 2 D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Purpureocillium lilacinum HKS-124 2 D-glucose
-
 ?
3.2.1.20 kojibiose + maltose transglycosylation activity Purpureocillium lilacinum 4-O-alpha-kojibiosyl-D-glucose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Purpureocillium lilacinum maltopentaose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Purpureocillium lilacinum D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Purpureocillium lilacinum 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Purpureocillium lilacinum HKS-124 2 D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Purpureocillium lilacinum maltotriose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Purpureocillium lilacinum maltose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Purpureocillium lilacinum HKS-124 maltose + D-glucose
-
 ?
3.2.1.20 additional information the enzyme has high transglucosylation activity synthesizing oligosaccharides containing alpha-1,3- and alpha-1,2-linkages, overview, 4-nitrophenyl alpha-D-glucopyranoside and isomaltose are poor substrates, no activity with trehalose, sucrose, dextran, cellobiose, pullulan, inullin, and cyclodextrin Purpureocillium lilacinum ?
-
 ?
3.2.1.20 additional information the enzyme has high transglucosylation activity synthesizing oligosaccharides containing alpha-1,3- and alpha-1,2-linkages, overview, 4-nitrophenyl alpha-D-glucopyranoside and isomaltose are poor substrates, no activity with trehalose, sucrose, dextran, cellobiose, pullulan, inullin, and cyclodextrin Purpureocillium lilacinum HKS-124 ?
-
 ?
3.2.1.20 nigerose + H2O best substrate Purpureocillium lilacinum 2 D-glucose
-
 ?
3.2.1.20 nigerose + H2O best substrate Purpureocillium lilacinum HKS-124 2 D-glucose
-
 ?
3.2.1.20 nigerose + maltose transglycosylation activity Purpureocillium lilacinum 4-O-alpha-nigerosyl-D-glucose + D-glucose
-
 ?
3.2.1.20 starch + H2O soluble starch Purpureocillium lilacinum D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 54000 Purpureocillium lilacinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 65
-
-
 Purpureocillium lilacinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5
-
-
 Purpureocillium lilacinum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.20 Purpureocillium lilacinum isoelectric focusing 4.7 4.6