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Literature summary extracted from

  • Saratani, Y.; Uheda, E.; Yamamoto, H.; Nishimura, A.; Yoshizako, F.
    Purification and properties of a carbonyl reductase involved in stereoselective reduction of ethyl 4-chloro-3-oxobutanoate from Cylindrocarpon sclerotigenum IFO 31855 (2003), Biosci. Biotechnol. Biochem., 67, 1417-1420.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.184 synthesis enzyme might be useful for production of ethyl (S)-4-chloro-3-hydroxybutanoate, which is used in the synthesis of pharmacologically and biologically important compounds Cylindrocarpon sclerotigenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 2,4-dinitrophenol 19% inhibition at 0.1 mM Cylindrocarpon sclerotigenum
1.1.1.184 Cd2+ 16% inhibition at 1 mM Cylindrocarpon sclerotigenum
1.1.1.184 Cu2+ 46% inhibition at 1 mM Cylindrocarpon sclerotigenum
1.1.1.184 dicoumarol 42% inhibition at 0.3 mM Cylindrocarpon sclerotigenum
1.1.1.184 DTNB 56% inhibition at 0.05 mM Cylindrocarpon sclerotigenum
1.1.1.184 EDTA 30% inhibition at 1 mM Cylindrocarpon sclerotigenum
1.1.1.184 Hg2+ complete inhibition at 1 mM Cylindrocarpon sclerotigenum
1.1.1.184 additional information no inhibition by diphenylhydantoin, N-ethylmaleimide, p-chloromercuribenzoate, PMSF, Mg2+, and Zn2+ Cylindrocarpon sclerotigenum
1.1.1.184 quercetin 16% inhibition at 0.1 mM Cylindrocarpon sclerotigenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 24800
-
 x * 24800, SDS-PAGE Cylindrocarpon sclerotigenum
1.1.1.184 68000
-
 gel filtration Cylindrocarpon sclerotigenum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Cylindrocarpon sclerotigenum
-
 enzyme CSCR1, strain IFO 31855
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 by sequential column chromatographies involving DEAE ion exchange, phenyl resin, and hydroxylapatite chromatography Cylindrocarpon sclerotigenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 125.3
-
 purified enzyme Cylindrocarpon sclerotigenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 2,3-butanedione + NADPH
-
 Cylindrocarpon sclerotigenum ? + NADP+
-
 ir
1.1.1.184 2,3-pentanedione + NADPH + H+ low activity Cylindrocarpon sclerotigenum ? + NADP+
-
 ir
1.1.1.184 D-xylose + NADPH very low activity Cylindrocarpon sclerotigenum ? + NADP+
-
 ir
1.1.1.184 ethyl 4-chloro-3-oxobutanoate + NADPH + H+ highly stereoselective reduction Cylindrocarpon sclerotigenum (S)-ethyl 4-chloro-3-hydroxybutanoate + NADP+
-
 ir
1.1.1.184 ethylpyruvate + NADPH low activity Cylindrocarpon sclerotigenum ? + NADP+
-
 ir
1.1.1.184 hydroxyacetone + NADPH low activity Cylindrocarpon sclerotigenum ? + NADP+
-
 ir
1.1.1.184 additional information enzyme shows highly restricted substrate specificity, overview Cylindrocarpon sclerotigenum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 oligomer x * 24800, SDS-PAGE Cylindrocarpon sclerotigenum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase
-
 Cylindrocarpon sclerotigenum
1.1.1.184 CSCR1
-
 Cylindrocarpon sclerotigenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 35
-
-
 Cylindrocarpon sclerotigenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 6.5
-
-
 Cylindrocarpon sclerotigenum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 additional information no activity with NADH Cylindrocarpon sclerotigenum
1.1.1.184 NADPH dependent on, no reverse reaction with NADP+ as cofactor Cylindrocarpon sclerotigenum