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Literature summary extracted from

  • Tanaka, Y.; Aki, T.; Hidaka, Y.; Furuya, Y.; Kawamoto, S.; Shigeta, S.; Ono, K.; Suzuki, O.
    Purification and characterization of a novel fungal alpha-glucosidase from Mortierella alliacea with high starch-hydrolytic activity (2002), Biosci. Biotechnol. Biochem., 66, 2415-2423.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information kinetics Mortierella alliacea
3.2.1.20 2.4
-
 maltotetraose pH 5.0, 37°C Mortierella alliacea
3.2.1.20 2.7
-
 maltotriose pH 5.0, 37°C Mortierella alliacea
3.2.1.20 2.8
-
 maltopentaose pH 5.0, 37°C Mortierella alliacea
3.2.1.20 2.9
-
 maltohexaose pH 5.0, 37°C Mortierella alliacea
3.2.1.20 3.5
-
 maltose pH 5.0, 37°C Mortierella alliacea
3.2.1.20 4.9
-
 maltoheptaose pH 5.0, 37°C Mortierella alliacea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 extracellular
-
 Mortierella alliacea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 31000
-
 1 * 61000 + 1 * 31000, SDS-PAGE Mortierella alliacea
3.2.1.20 61000
-
 1 * 61000 + 1 * 31000, SDS-PAGE Mortierella alliacea
3.2.1.20 92000
-
 gel filtration Mortierella alliacea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 additional information Mortierella alliacea the organism does not possess alpha-amylase activity ?
-
 ?
3.2.1.20 additional information Mortierella alliacea YN-15 the organism does not possess alpha-amylase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Mortierella alliacea
-
-
-
3.2.1.20 Mortierella alliacea YN-15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 from culture supernatant, 33fold by ethanol precipitation and 4 chromatographic steps Mortierella alliacea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 1
-
 purified enzyme, substrate isomaltose Mortierella alliacea
3.2.1.20 3.4
-
 purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside Mortierella alliacea
3.2.1.20 5.3
-
 purified enzyme, substrate maltitol Mortierella alliacea
3.2.1.20 7
-
 purified enzyme, substrate kojibiose Mortierella alliacea
3.2.1.20 8.3
-
 purified enzyme, substrate amylose Mortierella alliacea
3.2.1.20 26.1
-
 purified enzyme, substrate nigerose Mortierella alliacea
3.2.1.20 29.8
-
 purified enzyme, substrate maltose Mortierella alliacea
3.2.1.20 30.2
-
 purified enzyme, substrate glycogen Mortierella alliacea
3.2.1.20 34.3
-
 purified enzyme, substrate amylopectin Mortierella alliacea
3.2.1.20 42.9
-
 purified enzyme, substrate soluble starch Mortierella alliacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Mortierella alliacea 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 amylopectin + H2O high activity Mortierella alliacea ?
-
 ?
3.2.1.20 amylose + H2O n = 18 Mortierella alliacea alpha-D-glucose
-
 ?
3.2.1.20 D-glucose + ethanol transglucosylation activity in presence of ethanol with several substrates, e.g. maltose, kojibiose, nigerose, glycogen, starch, overview, the enzyme favors the forward reaction Mortierella alliacea ethyl-alpha-D-glucoside
-
 r
3.2.1.20 glycogen + H2O high activity Mortierella alliacea alpha-D-glucose
-
 ?
3.2.1.20 isomaltose + H2O low activity Mortierella alliacea 2 D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Mortierella alliacea alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Mortierella alliacea YN-15 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltitol + H2O
-
 Mortierella alliacea ?
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Mortierella alliacea maltohexaose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Mortierella alliacea maltopentaose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Mortierella alliacea D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O maltose is a glucosyl donor for transglycosylation activity, high activity Mortierella alliacea 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O maltose is a glucosyl donor for transglycosylation activity, high activity Mortierella alliacea YN-15 2 D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Mortierella alliacea maltotriose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Mortierella alliacea maltose + D-glucose
-
 ?
3.2.1.20 additional information the organism does not possess alpha-amylase activity Mortierella alliacea ?
-
 ?
3.2.1.20 additional information dextran, pullulan, ethyl-alpha-D-glucoside, sucrose, and trehalose are poor substrates Mortierella alliacea ?
-
 ?
3.2.1.20 additional information the organism does not possess alpha-amylase activity Mortierella alliacea YN-15 ?
-
 ?
3.2.1.20 additional information dextran, pullulan, ethyl-alpha-D-glucoside, sucrose, and trehalose are poor substrates Mortierella alliacea YN-15 ?
-
 ?
3.2.1.20 nigerose + H2O high activity Mortierella alliacea 2 alpha-D-glucose
-
 ?
3.2.1.20 nigerose + H2O high activity Mortierella alliacea YN-15 2 alpha-D-glucose
-
 ?
3.2.1.20 starch + H2O soluble starch, high activity, best substrate Mortierella alliacea alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 dimer 1 * 61000 + 1 * 31000, SDS-PAGE Mortierella alliacea
3.2.1.20 More the subunits are stably aggregated to each other even at high salt concentrations of 0.5 M Mortierella alliacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45 50 formation of ethyl-alpha-D-glucose Mortierella alliacea
3.2.1.20 55
-
 substrate maltose Mortierella alliacea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information
-
 Mortierella alliacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5
-
 formation of ethyl-alpha-D-glucose Mortierella alliacea
3.2.1.20 5 6 substrate maltose Mortierella alliacea