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Literature summary extracted from

  • Suzuki, K.; Sugawara, N.; Suzuki, M.; Uchiyama, T.; Katouno, F.; Nikaidou, N.; Watanabe, T.
    Chitinases A, B, and C1 of Serratia marcescens 2170 produced by recombinant Escherichia coli: enzymatic properties and synergism on chitin degradation (2002), Biosci. Biotechnol. Biochem., 66, 1075-1083.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Serratia marcescens
-
2170
-
3.2.1.14 Serratia marcescens 2170
-
2170
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O isoform ChiA is the most effective enzyme toward insoluble chitin, isoform ChiC1 is the most effective enzyme toward soluble chitin, ChiA, ChiB and ChiC1 hydrolyze water-soluble chitin very efficiently but powdered chitin is a very poor substrate Serratia marcescens N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine (GlcNAc)2 is almost exclusively generated from ChiA, ChiB and ChiC1, ChiA produced as minor products GlcNAc and (GlcNAc)3, ChiB produced as minor product only (GlcNAc)3, ChiC1 produced as minor product only GlcNAc ?
3.2.1.14 chitin + H2O isoform ChiA is the most effective enzyme toward insoluble chitin, isoform ChiC1 is the most effective enzyme toward soluble chitin, ChiA, ChiB and ChiC1 hydrolyze water-soluble chitin very efficiently but powdered chitin is a very poor substrate Serratia marcescens 2170 N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine (GlcNAc)2 is almost exclusively generated from ChiA, ChiB and ChiC1, ChiA produced as minor products GlcNAc and (GlcNAc)3, ChiB produced as minor product only (GlcNAc)3, ChiC1 produced as minor product only GlcNAc ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Serratia marcescens N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose ChiA almost exclusively generates (GlcNAc)2 and (GlcNAc)4, ChiB and ChiC1 generated all three products ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Serratia marcescens 2170 N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose ChiA almost exclusively generates (GlcNAc)2 and (GlcNAc)4, ChiB and ChiC1 generated all three products ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
ChiA and ChiB Serratia marcescens
3.2.1.14 65 70 ChiC1 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 30
-
ChiC1: 40% of activity remained, ChiA and ChiB: 10% of activity remained Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 10
-
Serratia marcescens