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Literature summary extracted from

  • Tsujibo, H.; Takada, C.; Tsuji, A.; Kosaka, M.; Miyamoto, K.; Inamori, Y.
    Cloning, sequencing, and expression of the gene encoding an intracellular beta-D-xylosidase from Streptomyces thermoviolaceus OPC-520 (2001), Biosci. Biotechnol. Biochem., 65, 1824-1831.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.37 dithiothreitol 1 mM, 9% activation Streptomyces thermoviolaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 AgNO3 1 mM, 64% inhibition Streptomyces thermoviolaceus
3.2.1.37 CdCl2 1 mM, 82% inhibition Streptomyces thermoviolaceus
3.2.1.37 CuCl2 1 mM, 83% inhibition Streptomyces thermoviolaceus
3.2.1.37 HgCl2 1 mM, 79% inhibition Streptomyces thermoviolaceus
3.2.1.37 NiCl2 1 mM, 70% inhibition Streptomyces thermoviolaceus
3.2.1.37 p-chloro-mercuribenzoate 1 mM, 80% inhibition Streptomyces thermoviolaceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 CaCl2 1 mM, 35% activation Streptomyces thermoviolaceus
3.2.1.37 CoCl2 1 mM, 42% activation Streptomyces thermoviolaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 82000
-
 1 * 82000, SDS-PAGE Streptomyces thermoviolaceus
3.2.1.37 83000
-
 gel filtration Streptomyces thermoviolaceus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Streptomyces thermoviolaceus Q76BU6 OPC-520, growth on xylan or xylooligosaccharides
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 partial Streptomyces thermoviolaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O 2.5% of the acticvity with p-nitrophenyl-beta-D-xylopyranoside Streptomyces thermoviolaceus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.37 p-nitrophenyl-beta-D-arabinofuranoside + H2O 8.8% of the acticvity with p-nitrophenyl-beta-D-xylopyranoside Streptomyces thermoviolaceus p-nitrophenol + beta-D-arabinofuranose
-
 ?
3.2.1.37 p-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Streptomyces thermoviolaceus p-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 monomer 1 * 82000, SDS-PAGE Streptomyces thermoviolaceus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 40 50
-
 Streptomyces thermoviolaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 6 7
-
 Streptomyces thermoviolaceus