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Literature summary extracted from

  • Ha, S.B.; Lee, D.E.; Lee, H.J.; Song, S.J.; Back, K.
    Activities of soluble and microsomal farnesyl diphosphatases in Datura stramonium (2003), Biol. Plant., 47, 477-479.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.7.1 ATP 0.05 mM, 8% activation of soluble FDPase activity Datura stramonium
3.1.7.1 isopentenyl diphosphate 20% activation of soluble FDPase activity at 0.1 mM and of microsomal FDPase activity at 0.02 mM Datura stramonium
3.1.7.1 p-nitrophenyl phosphate 0.05 mM, 10% activation of soluble FDPase activity, 20% activation of microsomal FDPase activity Datura stramonium
3.1.7.6 ATP 0.05, 8% stimulation Datura stramonium
3.1.7.6 isopentenyl diphosphate 20% stimulation of soluble enzyme at 0.1 mM, 20% stimulation of microsomal enzyme at 0.02 mM Datura stramonium
3.1.7.6 p-nitrophenyl phosphate 0.05, 10% stimulation Datura stramonium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.7.1 geranyl diphosphate 0.1 mM: 16% inhibition of soluble FDPase activity, 0.05 mM: 26% inhibition of microsomal FDPase activity, competitive Datura stramonium
3.1.7.1 geranylgeranyl diphosphate 0.05 mM, 16% inhibition of soluble and 38% inhibition of microsomal FDPase activity, competitive Datura stramonium
3.1.7.1 additional information not inhibited by ATP or p-nitrophenyl phosphate Datura stramonium
3.1.7.6 geranyl diphosphate 16% inhibition of soluble enzyme at 0.1 mM. 26% inhibition of microsomal enzyme at 0.05 mM Datura stramonium
3.1.7.6 geranylgeranyl diphosphate 0.05 mM, 16% inhibition of soluble enzyme, 38% inhibition of microsomal enzyme Datura stramonium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.7.1 microsome FDPase is constitutively expressed in the soluble and microsomal fractions, they may be distinct enzymes Datura stramonium
-
-
3.1.7.1 soluble FDPase is constitutively expressed in the soluble and microsomal fractions, they may be distinct enzymes Datura stramonium
-
-
3.1.7.6 microsome constitutively expressed at a high level in the soluble and the microsomal fraction Datura stramonium
-
-
3.1.7.6 soluble constitutively expressed at a high level in the soluble and the microsomal fraction Datura stramonium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.7.1 additional information Mg2+-independent Datura stramonium
3.1.7.6 additional information Mg2+-independent enzyme Datura stramonium

Organism

EC Number Organism UniProt Comment Textmining
3.1.7.1 Datura stramonium
-
-
-
3.1.7.6 Datura stramonium
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.7.1 flower highest soluble FDPase activity in fruit and flower Datura stramonium
-
3.1.7.1 fruit highest soluble FDPase activity in fruit and flower Datura stramonium
-
3.1.7.1 leaf lower soluble FDPase activity than in fruit and flower Datura stramonium
-
3.1.7.1 root lower soluble FDPase activity than in fruit and flower, highest microsomal FDPase activity Datura stramonium
-
3.1.7.6 flower soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf Datura stramonium
-
3.1.7.6 fruit soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf Datura stramonium
-
3.1.7.6 leaf soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf Datura stramonium
-
3.1.7.6 root soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf. Root has the highest level of microsomal enzyme Datura stramonium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.7.1 additional information
-
-
 Datura stramonium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.7.1 farnesyl diphosphate + H2O
-
 Datura stramonium farnesol + diphosphate
-
 ?
3.1.7.6 (2E,6E)-farnesyl diphosphate + H2O
-
 Datura stramonium (2E,6E)-farnesol + diphosphate diphosphate not determined ?

Synonyms

EC Number Synonyms Comment Organism
3.1.7.1 farnesyl diphosphatase
-
 Datura stramonium
3.1.7.1 FDPase
-
 Datura stramonium
3.1.7.1 prenol pyrophosphatase
-
 Datura stramonium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.7.1 35
-
 assay at Datura stramonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.7.1 5.2
-
 microsomal FDPase Datura stramonium
3.1.7.1 6
-
 soluble FDPase Datura stramonium
3.1.7.6 5.2
-
 microsomal enzyme Datura stramonium
3.1.7.6 6
-
 soluble enzyme Datura stramonium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.7.6 4.5 6.8 pH 4.5: about 55% of maximal activity, pH 6.8: about 60% of maximal activit, soluble enzyme Datura stramonium
3.1.7.6 5 6.8 pH 5.0: about 60% of maximal activity, pH 6.8: about 65% of maximal activit, microsomal enzyme Datura stramonium

Expression

EC Number Organism Comment Expression
3.1.7.6 Datura stramonium constitutively expressed at a high level in the soluble and the microsomal fraction up