Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ikegami, F.; Matsunae, K.; Hisamitsu, M.; Kurihara, T.; Yamamoto, T.; Murakoshi, I.
    Purification and properties of a plant beta-D-glucuronidase from Scutellaria root (1995), Biol. Pharm. Bull., 18, 1531-1534.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.167 Ag+ 0.3 mM, 20-25% inhibition Scutellaria baicalensis
3.2.1.167 Ca2+ 0.3 mM, about 15% inhibition Scutellaria baicalensis
3.2.1.167 Cu2+ 0.3 mM, 20-25% inhibition Scutellaria baicalensis
3.2.1.167 D-glucose 10 mM, 15% inhibition Scutellaria baicalensis
3.2.1.167 D-glucuronate 10 mM, 15% inhibition Scutellaria baicalensis
3.2.1.167 additional information 2-mercaptoethanol has no influence on activity up to 10 mM Scutellaria baicalensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.167 0.038
-
 5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate
-
 Scutellaria baicalensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.167 55000
-
 4 * 55000, SDS-PAGE Scutellaria baicalensis
3.2.1.167 200000 220000 gel filtration Scutellaria baicalensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.167 Scutellaria baicalensis
-
 Georg
-
3.2.1.167 Scutellaria baicalensis Georg
-
 Georg
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.167
-
 Scutellaria baicalensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.167 root
-
 Scutellaria baicalensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.167 114
-
-
 Scutellaria baicalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ?
3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis Georg 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ?
3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis 5,7-dihydroxy-8-methoxyflavone + D-glucuronate
-
 ?
3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georg 5,7-dihydroxy-8-methoxyflavone + D-glucuronate
-
 ?
3.2.1.167 additional information the enzyme is not able to cleave the bond between the two glucuronic acid moieties in glycyrrhizin (glycyrrhetic acid 3-O-D-diglucuronide), the enzyme is not capable of hydrolyzing glucosides, luteolin-7-glucoside and apigenin-7-O-glucoside Scutellaria baicalensis ?
-
 ?
3.2.1.167 additional information the enzyme is not able to cleave the bond between the two glucuronic acid moieties in glycyrrhizin (glycyrrhetic acid 3-O-D-diglucuronide), the enzyme is not capable of hydrolyzing glucosides, luteolin-7-glucoside and apigenin-7-O-glucoside Scutellaria baicalensis Georg ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.167 tetramer 4 * 55000, SDS-PAGE Scutellaria baicalensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.167 baicalinase
-
 Scutellaria baicalensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.167 40 50
-
 Scutellaria baicalensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.167 4.7
-
 in 50 mM Na-citrate buffer Scutellaria baicalensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.167 Scutellaria baicalensis chromatofocusing
-
 5.4