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Literature summary extracted from

  • Kim, D.H.; Jin, Y.H.; Jung, E.A.; Han, M.J.; Kobashi, K.
    Purification and characterization of beta-glucuronidase from Escherichia coli HGU-3, a human intestinal bacterium (1995), Biol. Pharm. Bull., 18, 1184-1188.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.31 medicine when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 CaCl2 2 mM, 14.8% inhibition Escherichia coli
3.2.1.31 D-glucuronate IC50: 7.5 mM, competitive Escherichia coli
3.2.1.31 FeCl2 2 mM,33.1% inhibition Escherichia coli
3.2.1.31 glycyrrhizin IC50: 0.08 mM, competitive Escherichia coli
3.2.1.31 MgCl2 2 mM, 24.8% inhibition Escherichia coli
3.2.1.31 MnCl2 2 mM, 31.9% inhibition Escherichia coli
3.2.1.31 NEM IC50: 0.09 mM Escherichia coli
3.2.1.31 NiCl2 2 mM, 52% inhibition Escherichia coli
3.2.1.31 p-chloromercuriphenylsulfonic acid IC50: 0.04 mM Escherichia coli
3.2.1.31 p-nitrophenol IC50: 0.3 mM, competitive Escherichia coli
3.2.1.31 saccharic acid 1,4-lactone IC50: 0.09 mM, competitive Escherichia coli
3.2.1.31 silybin IC50: 1 mM, non-competitive Escherichia coli
3.2.1.31 TLCK IC50: 0.3 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.22
-
p-nitrophenyl-beta-D-glucuronide pH 7.0, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 72000
-
4 * 72000, SDS-PAGE Escherichia coli
3.2.1.31 290000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.31 additional information Escherichia coli when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed ?
-
?
3.2.1.31 additional information Escherichia coli HGU-3 when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Escherichia coli
-
HGU-3
-
3.2.1.31 Escherichia coli HGU-3
-
HGU-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31
-
Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 17.78
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 bilirubin diglucuronide + H2O
-
Escherichia coli bilirubin + D-glucuronate
-
?
3.2.1.31 bilirubin diglucuronide + H2O
-
Escherichia coli HGU-3 bilirubin + D-glucuronate
-
?
3.2.1.31 additional information when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed Escherichia coli ?
-
?
3.2.1.31 additional information when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed Escherichia coli HGU-3 ?
-
?
3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O
-
Escherichia coli p-nitrophenol + D-glucuronate
-
?
3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O
-
Escherichia coli HGU-3 p-nitrophenol + D-glucuronate
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 tetramer 4 * 72000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 6.5
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.31 5 8.5 pH 5.0: about 45% of maximal activity, pH 8.5: about 50% of maximal activity Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.31 Escherichia coli chromatofocusing
-
4.9

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.31 0.04
-
IC50: 0.04 mM Escherichia coli p-chloromercuriphenylsulfonic acid
3.2.1.31 0.08
-
IC50: 0.08 mM, competitive Escherichia coli glycyrrhizin
3.2.1.31 0.09
-
IC50: 0.09 mM Escherichia coli NEM
3.2.1.31 0.09
-
IC50: 0.09 mM, competitive Escherichia coli saccharic acid 1,4-lactone
3.2.1.31 0.3
-
IC50: 0.3 mM Escherichia coli TLCK
3.2.1.31 0.3
-
IC50: 0.3 mM, competitive Escherichia coli p-nitrophenol
3.2.1.31 1
-
IC50: 1 mM, non-competitive Escherichia coli silybin
3.2.1.31 7.5
-
IC50: 7.5 mM, competitive Escherichia coli D-glucuronate