EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.31 | medicine | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.31 | CaCl2 | 2 mM, 14.8% inhibition | Escherichia coli | |
3.2.1.31 | D-glucuronate | IC50: 7.5 mM, competitive | Escherichia coli | |
3.2.1.31 | FeCl2 | 2 mM,33.1% inhibition | Escherichia coli | |
3.2.1.31 | glycyrrhizin | IC50: 0.08 mM, competitive | Escherichia coli | |
3.2.1.31 | MgCl2 | 2 mM, 24.8% inhibition | Escherichia coli | |
3.2.1.31 | MnCl2 | 2 mM, 31.9% inhibition | Escherichia coli | |
3.2.1.31 | NEM | IC50: 0.09 mM | Escherichia coli | |
3.2.1.31 | NiCl2 | 2 mM, 52% inhibition | Escherichia coli | |
3.2.1.31 | p-chloromercuriphenylsulfonic acid | IC50: 0.04 mM | Escherichia coli | |
3.2.1.31 | p-nitrophenol | IC50: 0.3 mM, competitive | Escherichia coli | |
3.2.1.31 | saccharic acid 1,4-lactone | IC50: 0.09 mM, competitive | Escherichia coli | |
3.2.1.31 | silybin | IC50: 1 mM, non-competitive | Escherichia coli | |
3.2.1.31 | TLCK | IC50: 0.3 mM | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.31 | 0.22 | - |
p-nitrophenyl-beta-D-glucuronide | pH 7.0, 37°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.31 | 72000 | - |
4 * 72000, SDS-PAGE | Escherichia coli |
3.2.1.31 | 290000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.31 | additional information | Escherichia coli | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | ? | - |
? | |
3.2.1.31 | additional information | Escherichia coli HGU-3 | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.31 | Escherichia coli | - |
HGU-3 | - |
3.2.1.31 | Escherichia coli HGU-3 | - |
HGU-3 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.31 | - |
Escherichia coli |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.31 | 17.78 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.31 | bilirubin diglucuronide + H2O | - |
Escherichia coli | bilirubin + D-glucuronate | - |
? | |
3.2.1.31 | bilirubin diglucuronide + H2O | - |
Escherichia coli HGU-3 | bilirubin + D-glucuronate | - |
? | |
3.2.1.31 | additional information | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | Escherichia coli | ? | - |
? | |
3.2.1.31 | additional information | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | Escherichia coli HGU-3 | ? | - |
? | |
3.2.1.31 | p-nitrophenyl-beta-D-glucuronide + H2O | - |
Escherichia coli | p-nitrophenol + D-glucuronate | - |
? | |
3.2.1.31 | p-nitrophenyl-beta-D-glucuronide + H2O | - |
Escherichia coli HGU-3 | p-nitrophenol + D-glucuronate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.31 | tetramer | 4 * 72000, SDS-PAGE | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.31 | 6.5 | - |
- |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.31 | 5 | 8.5 | pH 5.0: about 45% of maximal activity, pH 8.5: about 50% of maximal activity | Escherichia coli |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.31 | Escherichia coli | chromatofocusing | - |
4.9 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.31 | 0.04 | - |
IC50: 0.04 mM | Escherichia coli | p-chloromercuriphenylsulfonic acid | |
3.2.1.31 | 0.08 | - |
IC50: 0.08 mM, competitive | Escherichia coli | glycyrrhizin | |
3.2.1.31 | 0.09 | - |
IC50: 0.09 mM | Escherichia coli | NEM | |
3.2.1.31 | 0.09 | - |
IC50: 0.09 mM, competitive | Escherichia coli | saccharic acid 1,4-lactone | |
3.2.1.31 | 0.3 | - |
IC50: 0.3 mM | Escherichia coli | TLCK | |
3.2.1.31 | 0.3 | - |
IC50: 0.3 mM, competitive | Escherichia coli | p-nitrophenol | |
3.2.1.31 | 1 | - |
IC50: 1 mM, non-competitive | Escherichia coli | silybin | |
3.2.1.31 | 7.5 | - |
IC50: 7.5 mM, competitive | Escherichia coli | D-glucuronate |