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Literature summary extracted from

  • Okuda, K.; Nishiyama, Y.; Morita, E.H.; Hayashi, H.
    Identification and characterization of NuhA, a novel Nudix hydrolase specific for ADP-ribose in the cyanobacterium Synechococcus sp. PCC 7002 (2004), Biochim. Biophys. Acta, 1699, 245-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13
-
 Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.094
-
 ADPribose pH 9.5, 37°C Synechococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ divalent cation required, Mg2+ is most effective Synechococcus sp.
3.6.1.13 Mn2+ divalent cation required, Mn2+ results in 22% of the activity observed in presence of Mg2+ Synechococcus sp.
3.6.1.13 Zn2+ divalent cation required, Zn2+ results in 9% of the activity observed in presence of Mg2+ Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.13 26735
-
 6 * 26735, calculation from nucleotide sequence Synechococcus sp.
3.6.1.13 28000
-
 6 * 28000, SDS-PAGE Synechococcus sp.
3.6.1.13 166000
-
 gel filtration Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Synechococcus sp. Q83ZD0 PCC 7002
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13
-
 Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-glucose + H2O 9% of the activity with ADPribose Synechococcus sp. AMP + alpha-D-glucose 1-phosphate
-
 ?
3.6.1.13 ADPribose + H2O
-
 Synechococcus sp. AMP + D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.13 hexamer 6 * 28000, SDS-PAGE Synechococcus sp.
3.6.1.13 hexamer 6 * 26735, calculation from nucleotide sequence Synechococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 NuhA
-
 Synechococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.13 1.8
-
 ADPribose pH 9.5, 37°C Synechococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 9.5
-
-
 Synechococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.13 8 10 pH 8.0: about 40% of maximal activity, pH 10.0: about 60% of maximal activity Synechococcus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.13 Synechococcus sp.
-
-
 6