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Literature summary extracted from

  • Inose, K.; Fujikawa, M.; Yamazaki, T.; Kojima, K.; Sode, K.
    Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli (2003), Biochim. Biophys. Acta, 1645, 133-138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 DNA and amino acid sequence determination and analysis, expression of the catalytic alpha-subunit in Escherichia coli as active water-soluble protein Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 additional information no inhibition by EDTA and thiol reagents such as fluorescein 5-maleimide Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 2.8
-
 beta-D-glucose pH 7.0, 70°C Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.47 additional information enzyme does not contain weakly associated metal ions Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 60000
-
 x * 60000, recombinant catalytic alpha-subunit, SDS-PAGE Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Burkholderia cepacia Q8GQE7 FERM BP-7306 strain SM-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 recombinant catalytic alpha-subunit from Escherichia coli Burkholderia cepacia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 2000
-
 above, purified recombinant catalytic alpha-subunit, 40°C Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD(P)+
-
 Burkholderia cepacia D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir
1.1.1.47 D-galactose + NAD(P)+
-
 Burkholderia cepacia D-galactono-1,5-lactone + NAD(P)H
-
 ?
1.1.1.47 additional information Cys residues are not involved in the catalytic site Burkholderia cepacia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 ? x * 60000, recombinant catalytic alpha-subunit, SDS-PAGE Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 GDH
-
 Burkholderia cepacia
1.1.1.47 glucose dehydrogenase
-
 Burkholderia cepacia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 70
-
-
 Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 additional information
-
 thermostable enzyme Burkholderia cepacia
1.1.1.47 60
-
 30 min, stable Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 7
-
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 FAD the catalytic alpha-subunit contains a FAD binding motif at the N-terminal region Burkholderia cepacia
1.1.1.47 NAD+
-
 Burkholderia cepacia
1.1.1.47 NADH
-
 Burkholderia cepacia
1.1.1.47 NADP+
-
 Burkholderia cepacia
1.1.1.47 NADPH
-
 Burkholderia cepacia