EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.1.2 | overexpression of HoxE in Escherichia coli strain M15 | Synechocystis sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.1.2 | 0.089 | - |
reduced methyl viologen | pH 6.3, 60°C | Synechocystis sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | additional information | metalloenzyme | Synechocystis sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 18000 | - |
x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE | Synechocystis sp. |
1.12.1.2 | 21000 | - |
x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE | Synechocystis sp. |
1.12.1.2 | 375000 | - |
native dimeric assembly of the pentameric enzyme complex, gel filtration | Synechocystis sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | Synechocystis sp. | - |
H+ + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.1.2 | Synechocystis sp. | Q9Z354 | HoxE subunit from strain PCC 6301; strain PCC 6803, and strain PCC 6301 which is identical with Anacystis nidulans SAUG 1402-1 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.1.2 | enzyme is oxygen-labile | Synechocystis sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.1.2 | under anaerobic conditions, pentameric bidirectional hydrogenase complex HoxEFUYH to near homogeneity 647fold from strain PCC 6803 and 1290fold from strain PCC 6301, recombinant His-tagged HoxE from Escherichia coli | Synechocystis sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 15 | - |
native purified enzyme complex from strain PCC 6301, hydrogen-forming activity, cofactor reduced methyl viologen | Synechocystis sp. |
1.12.1.2 | 67 | - |
native purified enzyme complex from strain PCC 6803, hydrogen-forming activity, cofactor reduced methyl viologen | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD(P)+ | - |
Synechocystis sp. | H+ + NAD(P)H | - |
r | |
1.12.1.2 | H2 + NAD+ | - |
Synechocystis sp. | H+ + NADH | - |
r | |
1.12.1.2 | H2 + oxidized dithionite | - |
Synechocystis sp. | H+ + reduced dithionite | - |
r | |
1.12.1.2 | H2 + oxidized methyl viologen | - |
Synechocystis sp. | H+ + reduced methyl viologen | best cofactor | r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.1.2 | ? | x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE | Synechocystis sp. |
1.12.1.2 | More | the subunit HoxE, which is essential for the bidirectional hydrogenase activity, is included in a pentameric bidirectional hydrogenase complex HoxEFUYH in cyanobacteria, the complex forms dimer (HoxEFUYH)2, complex components are HoxE, HoxF, HoxH, HoxU, and HoxY, complex composition analysis, overview | Synechocystis sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.1.2 | HoxE | - |
Synechocystis sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 60 | - |
strain PCC 6803, hydrogen-forming activity | Synechocystis sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 75 | - |
inactivation above | Synechocystis sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 6.3 | - |
strain PCC 6803, hydrogen-forming activity | Synechocystis sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | additional information | methyl viologen or dithionite can act as electron donor/acceptor | Synechocystis sp. | |
1.12.1.2 | additional information | no activity with ferredoxin or FMN | Synechocystis sp. | |
1.12.1.2 | NAD(P)+ | - |
Synechocystis sp. | |
1.12.1.2 | NAD(P)H | NADH is 2times more active than NADPH | Synechocystis sp. |