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Literature summary extracted from

  • Takesue, Y.; Yokota, K.; Oda, S.; Takesue, S.
    Comparison of sucrase-free isomaltase with sucrase-isomaltase purified from the house musk shrew Suncus murinus (2001), Biochim. Biophys. Acta, 1544, 341-349.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.10 additional information the sucrase-free mutant lacks the entire sucrase domain and 69 amino acids of the C-terminus of isomaltase domain, the isomaltase function is unaffected by the mutation, sucrase function is missing Suncus murinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 7
-
maltose wild-type sucrase-isomaltase Suncus murinus
3.2.1.10 12.8
-
isomaltose wild-type sucrase-isomaltase Suncus murinus
3.2.1.10 13.6
-
isomaltose mutant isomaltase Suncus murinus
3.2.1.10 27.5
-
sucrose wild-type sucrase-isomaltase Suncus murinus
3.2.1.10 62.8
-
maltose mutant isomaltase Suncus murinus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.10 membrane
-
Suncus murinus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 103000
-
1 * 103000, mutant sucrase-free isomaltase, SDS-PAGE Suncus murinus
3.2.1.10 106000
-
1 * 106000, isomaltase subunit of the wild-type enzyme, + 1 * 115000, sucrase subunit of the wild-type enzyme, SDS-PAGE Suncus murinus
3.2.1.10 115000
-
1 * 106000, isomaltase subunit of the wild-type enzyme, + 1 * 115000, sucrase subunit of the wild-type enzyme, SDS-PAGE Suncus murinus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Suncus murinus
-
house musk shrew, contains 2 enzyme forms: a sucrase-free isomaltase and a sucrase-isomaltase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.10 glycoprotein enzyme, especially the mutant enzyme, contains probably carbohydrates with terminal L-fucose, determined by lectin affinity, overview Suncus murinus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10 sucrase-free isomaltase and a sucrase-isomaltase from a wild-type and a sucrase-deficient mutant line, from intestine, by temperature adsorption-desorption affinity chromatography on Sephadex gel Suncus murinus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.10 intestine
-
Suncus murinus
-
3.2.1.10 mucosa
-
Suncus murinus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.10 2.12
-
purified mutant sucrase-free isomaltase, maltase activity Suncus murinus
3.2.1.10 7.79
-
purified wild-type sucrase-isomaltase, sucrase activity Suncus murinus
3.2.1.10 8.08
-
purified wild-type sucrase-isomaltase, isomaltase activity Suncus murinus
3.2.1.10 12.3
-
purified mutant sucrase-free isomaltase, isomaltase activity Suncus murinus
3.2.1.10 14.5
-
purified wild-type sucrase-isomaltase, maltase activity Suncus murinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 amylose + H2O n = 18, low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 glycogen + H2O substrate from oyster, low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 isomaltose + H2O best substrate for the mutant isomaltase Suncus murinus 2 D-glucose
-
?
3.2.1.10 maltoheptaose + H2O low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 maltohexaose + H2O low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 maltopentaose + H2O
-
Suncus murinus ?
-
?
3.2.1.10 maltose + H2O best substrate of the wild-type sucrase-isomaltase Suncus murinus 2 D-glucose
-
?
3.2.1.10 maltotetraose + H2O low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 maltotriose + H2O low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 additional information substrate specificities of mutant isomaltase and wild-type sucrase-isomaltase, overview Suncus murinus ?
-
?
3.2.1.10 pullulan + H2O low activity Suncus murinus ?
-
?
3.2.1.10 soluble starch + H2O low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase Suncus murinus ?
-
?
3.2.1.10 sucrose + H2O wild-type sucrase-isomaltase, no activity with the mutant isomaltase Suncus murinus D-glucose + D-fructose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.10 dimer 1 * 106000, isomaltase subunit of the wild-type enzyme, + 1 * 115000, sucrase subunit of the wild-type enzyme, SDS-PAGE Suncus murinus
3.2.1.10 monomer 1 * 103000, mutant sucrase-free isomaltase, SDS-PAGE Suncus murinus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 isomaltase
-
Suncus murinus
3.2.1.10 sucrase-isomaltase
-
Suncus murinus