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Literature summary extracted from

  • Awotunde, O.S.; Sugajska, E.; Zolnierowicz, S.; Muszynska, G.
    Characterisation of two protein phosphatase 2A holoenzymes from maize seedlings (2000), Biochim. Biophys. Acta, 1480, 65-76.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.16 histone H1 increased activity Zea mays
3.1.3.16 polylysine increased activity Zea mays
3.1.3.16 protamine in the presence of ammonium sulfate Zea mays
3.1.3.16 protamine in the presence of ammoniumsulfate Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 okadaic acid IC50: 0.18 nM; IC50: 0.1 nM Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 38000
-
 alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE Zea mays
3.1.3.16 57000
-
 alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE Zea mays
3.1.3.16 57000
-
 x * 57000 + x * 66000, SDS-PAGE Zea mays
3.1.3.16 66000
-
 alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE Zea mays
3.1.3.16 66000
-
 x * 57000 + x * 66000, SDS-PAGE Zea mays
3.1.3.16 140000 160000 gel filtration Zea mays
3.1.3.16 180000 200000 gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 0.064
-
 after thiophosphorylase alpha-Sepharose purification step, with phosphorylated alpha phosphorylase as substrate and in the presence of 0.03 mg protamine and 16 mM ammonium sulfate Zea mays
3.1.3.16 0.12
-
 after thiophosphorylase alpha-Sepharose purification step, with phosphorylated alpha phosphorylase as substrate and in the presence of 0.03 mg protamine and 16 mM ammonium sulfate Zea mays
3.1.3.16 0.49
-
 with p-nitrophenyl phosphate as substrate Zea mays
3.1.3.16 1.12
-
 with p-nitrophenyl phosphate as substrate Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 p-nitrophenyl phosphate + H2O
-
 Zea mays p-nitrophenol + phosphate
-
 ?
3.1.3.16 phosphorylated phosphorylase alpha + H2O
-
 Zea mays phosphorylase alpha + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 ? x * 57000 + x * 66000, SDS-PAGE Zea mays
3.1.3.16 trimer alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 PP2AI
-
 Zea mays
3.1.3.16 PP2AII
-
 Zea mays

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.16 0.0000001
-
 IC50: 0.1 nM Zea mays okadaic acid
3.1.3.16 0.00000018
-
 IC50: 0.18 nM Zea mays okadaic acid