EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.9 | 2',5'-ADP | stimulates the cyclohydrolase reverse reaction, 2'-phosphate is involved | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.9 | expression of wild-type and mutant enzymes in Escherichia coli JM109 as C-terminally His-tagged enzymes | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.4.9 | R173A | site-directed mutagenesis, at least 500fold increased Km for NADP+ compared to the wild-type, 163fold increased activity in the forward and reduced activity in the reverse reaction of the cyclohydrolase, unaltered channeling efficiency | Homo sapiens |
3.5.4.9 | R173E | site-directed mutagenesis, no dehydrogenase forward reaction activity, no forward channeling | Homo sapiens |
3.5.4.9 | R173K | site-directed mutagenesis, at least 500fold increased Km for NADP+ compared to the wild-type, 46fold increased activity in the forward and reduced activity in the reverse reaction of the cyclohydrolase, unaltered channeling efficiency | Homo sapiens |
3.5.4.9 | S197A | site-directed mutagenesis, 20fold increased Km for NADP+ compared to the wild-type, reduced activity in the reverse reaction of the cyclohydrolase, no stimulation by 2',5'-ADP, unaltered channeling efficiency | Homo sapiens |
3.5.4.9 | S197D | site-directed mutagenesis, 8.5fold increased activity in the forward reaction of the cyclohydrolase, unaltered channeling efficiency | Homo sapiens |
3.5.4.9 | S197R | site-directed mutagenesis, 10fold increased Km for methenyltetrahydrofolate, reduced activity in the forward and reverse reaction of the cyclohydrolase, unaltered channeling efficiency | Homo sapiens |
3.5.4.9 | S197T | site-directed mutagenesis, slightly increased activity in the forward reaction of the cyclohydrolase, unaltered channeling efficiency | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.9 | additional information | - |
additional information | the His-tag does not alter the activity and kinetics of the enzymes | Homo sapiens | |
3.5.4.9 | 0.016 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant S197D, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.018 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.019 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197D | Homo sapiens | |
3.5.4.9 | 0.02 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant S197T, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.021 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant R173A and R173K | Homo sapiens | |
3.5.4.9 | 0.024 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant R173A, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.025 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197T | Homo sapiens | |
3.5.4.9 | 0.025 | - |
methenyltetrahydrofolate | forward reaction, recombinant wild-type enzyme | Homo sapiens | |
3.5.4.9 | 0.026 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant R173E | Homo sapiens | |
3.5.4.9 | 0.026 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant wild-type enzyme, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.028 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant S197R, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.031 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant R173E, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.038 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197A | Homo sapiens | |
3.5.4.9 | 0.043 | - |
10-formyltetrahydrofolate | reverse reaction, recombinant mutant S197A, substrate 10-formyltetrahydrofolate | Homo sapiens | |
3.5.4.9 | 0.23 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197R | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.9 | Homo sapiens | - |
bifunctional methenyltetrahydrofolate dehydrogenase/cyclohydrolase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.9 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by Ni2+-chelate affinity chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate | In eukaryotes, the enzyme occurs as a trifunctional enzyme that also has methylenetetrahydrofolate dehydrogenase (NADP+), EC 1.5.1.5, and formate-tetrahydrofolate ligase, EC 6.3.4.3, activity. In some prokaryotes, it occurs as a bifunctional enzyme, that also has methylenetetrahydrofolate dehydrogenase (NADP+), EC 1.5.1.5, activity or formiminotetrahydrofolate cyclodeaminase, EC 4.3.1.4, activity, residue Arg173 is critical for binding of NADP+ with Ser197 playing a supporting role, both residues interact with the 2'-phosphate of NADP+ | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.9 | additional information | - |
the His-tag does not alter the activity and kinetics of the enzymes, determination of methylenetetrahydrofolate dehydrogenase (NADP+), EC 1.5.1.5, activity, determination of forward channeling in percent | Homo sapiens |
3.5.4.9 | 3.4 | - |
forward reaction, recombinant mutant S197R, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
3.5.4.9 | 6.4 | - |
reverse reaction, recombinant mutant S197A, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 7.2 | - |
forward reaction, recombinant wild-type enzyme, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
3.5.4.9 | 7.3 | - |
reverse reaction, recombinant mutant S197R, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 7.6 | - |
reverse reaction, recombinant mutant S197A, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 8 | - |
reverse reaction, recombinant mutant R173A, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 9 | - |
reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 9.2 | - |
reverse reaction, recombinant mutant R173E, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 9.2 | - |
reverse reaction, recombinant mutant S197T, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 9.3 | - |
reverse reaction, recombinant mutant S197D, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 9.6 | - |
reverse reaction, recombinant wild-type enzyme, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 10.8 | - |
forward reaction, recombinant mutant S197T, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
3.5.4.9 | 10.8 | - |
reverse reaction, recombinant mutant S197D, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 12.8 | - |
reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 13.5 | - |
forward reaction, recombinant mutant S197A, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
3.5.4.9 | 15.9 | - |
reverse reaction, recombinant mutant S197T, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 22.4 | - |
reverse reaction, recombinant wild-type enzyme, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 22.8 | - |
reverse reaction, recombinant mutant S197R, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP | Homo sapiens |
3.5.4.9 | 61.6 | - |
forward reaction, recombinant mutant S197D, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
3.5.4.9 | 329 | - |
forward reaction, recombinant mutant R173K, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
3.5.4.9 | 1173 | - |
forward reaction, recombinant mutant R173A, substrate 5,10-methenyltetrahydrofolate | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | 5,10-methenyltetrahydrofolate 5-hydrolase (decyclizing), EC 3.5.4.9, activity of the enzyme | Homo sapiens | 10-formyltetrahydrofolate | - |
r | |
3.5.4.9 | 5,10-methylenetetrahydrofolate + NADP+ | methylenetetrahydrofolate dehydrogenase (NADP+), EC 1.5.1.5, activity of the enzyme | Homo sapiens | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
r | |
3.5.4.9 | additional information | the enzyme has a bifunctional DC domain that binds NADP+ and one folate molecule within a single catalytic cleft being part of a trifunctional enzyme, substrate channeling with high efficiency independent of NADP+ substrate binding efficiency | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.9 | methenyltetrahydrofolate dehydrogenase/cyclohydrolase | - |
Homo sapiens |
3.5.4.9 | methyleneH4folate cyclohydrolase | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.9 | 107 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197T | Homo sapiens | |
3.5.4.9 | 118 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197A | Homo sapiens | |
3.5.4.9 | 123 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant R173E | Homo sapiens | |
3.5.4.9 | 128 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant R173A | Homo sapiens | |
3.5.4.9 | 134 | - |
methenyltetrahydrofolate | forward reaction, recombinant wild-type enzyme | Homo sapiens | |
3.5.4.9 | 141 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant R173K | Homo sapiens | |
3.5.4.9 | 177 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197D | Homo sapiens | |
3.5.4.9 | 185 | - |
methenyltetrahydrofolate | forward reaction, recombinant mutant S197R | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.9 | 7.3 | - |
assay at | Homo sapiens |